Notes on the thiocyanate iron reaction : a modified procedure for the quantitative determination of iron in biological materials
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Date
Authors
Truter, G.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Pretoria : The Government Printer
Abstract
1. A modified procedure of the thiocyanate method for the micro-determination
of iron in biological materials is described.
2. Evidence is presented to show its efficiency in the presence of moderate
quantities of copper and relatively large concentrations of phosphate and
calcium.
3. Hydrochloric or sulphuric acid is preferable to nitric acid, which
should rather be avoided.
4. For colour stability it is essential to avoid recovered iso-amyl alcohol
previously used for either iron or copper determinations.
5. A detailed description of a wet combustion procedure is given.
6. Some data on the iron and copper contents of tissues are given.
7. It can be concluded that the proposed method not only satisfies the
need for a time-saving and simple procedure, but also conforms to the
requirements for accuracy and reliability, if the necessary precautionary
measures are taken.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Truter, GJ 1947, 'Notes on the thiocyanate iron reaction : a modified procedure for the quantitative determination of iron in biological materials’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 21, no. 2, pp. 305-315.