Effects of ω3- and ω6-polyunsaturated fatty acids on RANKL-induced osteoclast differentiation of RAW264.7 cells : a comparative in vitro study

dc.contributor.authorBoeyens, Jan Christoffel Antonie
dc.contributor.authorDeepak, Vishwa
dc.contributor.authorChua, Wei-Hang
dc.contributor.authorKruger, Marlena C.
dc.contributor.authorJoubert, Annie M.
dc.contributor.authorCoetzee, Magdalena
dc.contributor.emailannie.joubert@up.ac.zaen_US
dc.date.accessioned2014-08-15T12:28:35Z
dc.date.available2014-08-15T12:28:35Z
dc.date.issued2014-07
dc.description.abstractPolyunsaturated fatty acids (PUFAs) have been reported to have an anabolic effect on bone in vivo, but comparative studies to identify inhibitors of osteoclast formation amongst ω3- and ω6-PUFAs are still lacking. Here we assessed the effects of the ω3-PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and the ω6-PUFAs, arachidonic acid (AA) and γ-linolenic acid (GLA) on a RAW264.7 osteoclast differentiation model. The effects of PUFAs on RANKL-induced osteoclast formation were evaluated by counting tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells. PUFAs significantly inhibited RANKL-induced osteoclast formation in a dose-dependent manner with AA- and DHA-mediated inhibition being the strongest. Furthermore, RANKL-induced mRNA- and protein expression of the key osteoclastogenic genes cathepsin K and TRAP were inhibited by AA and more potently by DHA. Owing to the attenuated osteoclastogenesis by DHA and AA, actin ring formation and bone resorptive activity of these cells as evaluated on bone-mimetic plates were severely compromised. Hence, of the tested PUFAs, AA and DHA were found to be the most effective in inhibiting RANKL-induced osteoclast formation with the latter providing the strongest inhibitory effects. Collectively, the data indicates that these PUFAs may play an important role in regulating bone diseases characterized by excessive osteoclast activity.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipM. Coetzee received funding from the South African Medical Research Council (MRC) and the Research Committee (School of Medicine) of the University of Pretoria. V. Deepak is recipient of a Vice Chancellor’s postdoctoral fellowship from University of Pretoria.en_US
dc.description.urihttp://www.mdpi.com/journal/nutrientsen_US
dc.identifier.citationBoeyens, JCA, Deepak, V, Chua, WH, Kruger, MC, Joubert, AM, Coetzee, M 2014, 'Effects of ω3- and ω6-polyunsaturated fatty acids on RANKL-induced osteoclast differentiation of RAW264.7 cells: A comparative in vitro study', Nutrients, vol. 6, no. 7, pp. 2584-2601.
dc.identifier.issn2072-6643 (online)
dc.identifier.other10.3390/nu6072584
dc.identifier.urihttp://hdl.handle.net/2263/41350
dc.language.isoenen_US
dc.publisherMolecular Diversity Preservation Internationalen_US
dc.rights© Molecular Diversity Preservation Internationalen_US
dc.subjectOsteoclastsen_US
dc.subjectPolyunsaturated fatty acidsen_US
dc.subjectBone resorptionen_US
dc.subjectArachidonic aciden_US
dc.subjectGamma-linolenic aciden_US
dc.subjectEicosapentaenoic aciden_US
dc.subjectDocosahexaenoic aciden_US
dc.subjectRAW264.7 macrophagesen_US
dc.titleEffects of ω3- and ω6-polyunsaturated fatty acids on RANKL-induced osteoclast differentiation of RAW264.7 cells : a comparative in vitro studyen_US
dc.typeArticleen_US

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