The dynamic gut microbiota of zoophilic members of the Anopheles gambiae complex (Diptera: Culicidae)

dc.contributor.authorSingh, Ashmika
dc.contributor.authorAllam, Mushal
dc.contributor.authorKwenda, Stanford
dc.contributor.authorKhumalo, Zamantungwa Thobeka Happiness
dc.contributor.authorIsmail, Arshad
dc.contributor.authorOliver, Shüné
dc.date.accessioned2022-11-02T09:12:04Z
dc.date.available2022-11-02T09:12:04Z
dc.date.issued2022-01-27
dc.description.abstractThe gut microbiota of mosquitoes plays a critical role in the life history of the animal. There is a growing body of research characterising the gut microbiota of a range of mosquito species, but there is still a paucity of information on some members of the Anopheles gambiae complex. In this study, the gut microbiota of four laboratory strains were characterised. SENN (Anopheles arabiensis—insecticide susceptible major vector), SENN DDT (Anopheles arabiensis—insecticide resistant major vector), MAFUS (Anopheles merus—minor vector) and SANGWE (Anopheles quadriannulatus—non-vector) were used in this study. The microbiota of fourth instar larvae, 3-day old, 15-day old non-blood fed and 15-day old blood fed females were characterised by MALDI-TOF mass spectroscopy and 16 s rRNA gene sequencing by next generation sequencing. The four strains differed in species richness but not diversity. The major vectors differ in β-diversity from that of the minor and non-vectors. There was no difference in α- or β-diversity in 15 non-blood fed females and 15-day old females that had 3 blood meals before day 15. These differences may be related to a mixture of the effect of insecticide resistance phenotype as well as a potential relationship to vector competence to a limited extent. Bacterial diversity is affected by species and age. There is also a potential relationship between the differences in gut microbiota and capacity to transmit parasites. This genetic background of the mosquitoes, however, play a major role, and must be considered in this relationship.en_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.sponsorshipThe National Research Foundation of South Africa Competitive Support for Unrated Researchers and the National Health Laboratory Services Research Trust Development Grant.en_US
dc.description.urihttps://www.nature.com/srepen_US
dc.identifier.citationSingh, A., Allam, M., Kwenda, S. et al. The dynamic gut microbiota of zoophilic members of the Anopheles gambiae complex (Diptera: Culicidae). Scientific Reports 12, 1495 (2022). https://doi.org/10.1038/s41598-022-05437-y.en_US
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-022-05437-y
dc.identifier.urihttps://repository.up.ac.za/handle/2263/88100
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.rights© The Author(s) 2022. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectGut microbiotaen_US
dc.subjectZoophilic membersen_US
dc.subjectAnopheles gambiaeen_US
dc.subjectMicrobial communitiesen_US
dc.subjectEntomologyen_US
dc.subjectMicrobiologyen_US
dc.subjectZoologyen_US
dc.subjectMosquitoesen_US
dc.titleThe dynamic gut microbiota of zoophilic members of the Anopheles gambiae complex (Diptera: Culicidae)en_US
dc.typeArticleen_US

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