Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants
dc.contributor.author | Mashamba, Tebatso Gratitude | |
dc.contributor.author | Adeosun, Idowu Jesulayomi | |
dc.contributor.author | Baloyi, Itumeleng Tsebang | |
dc.contributor.author | Tshikalange, Thilivhali Emmanuel | |
dc.contributor.author | Cosa, Sekelwa | |
dc.contributor.email | sekelwa.cosa@up.ac.za | en_US |
dc.date.accessioned | 2022-11-10T05:34:50Z | |
dc.date.available | 2022-11-10T05:34:50Z | |
dc.date.issued | 2022-04-27 | |
dc.description.abstract | The crisis of antibiotic resistance necessitates the search of phytochemicals as potential antibacterial, anti-quorum sensing and antibiofilm forming agents. For the present study, fifteen (15) selected medicinal plants were evaluated to inhibit the biological activities of multi-drug resistant (MDR) pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis) associated with diabetic foot ulcer. Antibacterial activities revealed noteworthy minimum inhibitory concentration (MIC) values ≤1 mg/mL for thirteen (13) out of the sixty (60) plant extracts screened. The potent extracts included Euclea natalensis ethyl acetate (0.25 mg/mL), Aloe ferox methanol (0.5 mg/ml) and Warburgia salutaris aqueous (0.5 mg/mL) extracts. Chemical profiling of the active extracts using gas chromatography-mass spectrometry (GC-MS) identified neophytadiene, guanosine, squalene, cis megastigma-5,8-diene-4-one and sorbitol as prevalent compounds among the active extracts. Anti-quorum sensing activities of E. natalensis (ethyl acetate), A. ferox (methanol) and W. salutaris (aqueous) extracts ranged from 4.81 - 58.34% with E. natalensis (ethyl-acetate) showing the highest activity. Molecular docking against CviR protein showed selected compounds having high docking scores with sorbitol showing the highest score of -7.04 kcal/mol. Warburgia salutaris aqueous extract exhibited the highest biofilm inhibition (73%) against E. coli. Euclea natalensis, Aloe ferox and Warburgia salutaris compounds act as antagonist of N-acyl homoserine lactone (AHL) signaling, thus may serve as candidates in antipathogenic and antibiofilm phytomedicine development for MDR foot ulcer bacterial pathogens. | en_US |
dc.description.department | Biochemistry | en_US |
dc.description.department | Genetics | en_US |
dc.description.department | Microbiology and Plant Pathology | en_US |
dc.description.department | Plant Production and Soil Science | en_US |
dc.description.sponsorship | The South African National Research Foundation (NRF) Thuthuka Grant and the South African Medical Research Council-Self Initiated Research (SAMRC-SIR). | en_US |
dc.description.uri | https://www.cell.com/heliyon/home | en_US |
dc.identifier.citation | Mashamba, T.G., Adeosun, I.J., Baloyi, I.T., Tshikalange, E.T. & Cosa S. Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants. Heliyon. 2022 Apr 27;8(4):e09303. doi:10.1016/j.heliyon.2022.e09303. | en_US |
dc.identifier.issn | 2405-8440 (online) | |
dc.identifier.other | 10.1016/j.heliyon.2022.e09303 | |
dc.identifier.uri | https://repository.up.ac.za/handle/2263/88235 | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | © 2022 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en_US |
dc.subject | Aloe ferox | en_US |
dc.subject | Antiquorum sensing | en_US |
dc.subject | Biofilm inhibition | en_US |
dc.subject | Euclea natalensis | en_US |
dc.subject | Molecular modelling | en_US |
dc.subject | Multi-drug resistant pathogens | en_US |
dc.subject | Multidrug-resistant (MDR) | en_US |
dc.title | Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants | en_US |
dc.type | Article | en_US |