Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants

dc.contributor.authorMashamba, Tebatso Gratitude
dc.contributor.authorAdeosun, Idowu Jesulayomi
dc.contributor.authorBaloyi, Itumeleng Tsebang
dc.contributor.authorTshikalange, Thilivhali Emmanuel
dc.contributor.authorCosa, Sekelwa
dc.contributor.emailsekelwa.cosa@up.ac.zaen_US
dc.date.accessioned2022-11-10T05:34:50Z
dc.date.available2022-11-10T05:34:50Z
dc.date.issued2022-04-27
dc.description.abstractThe crisis of antibiotic resistance necessitates the search of phytochemicals as potential antibacterial, anti-quorum sensing and antibiofilm forming agents. For the present study, fifteen (15) selected medicinal plants were evaluated to inhibit the biological activities of multi-drug resistant (MDR) pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis) associated with diabetic foot ulcer. Antibacterial activities revealed noteworthy minimum inhibitory concentration (MIC) values ≤1 mg/mL for thirteen (13) out of the sixty (60) plant extracts screened. The potent extracts included Euclea natalensis ethyl acetate (0.25 mg/mL), Aloe ferox methanol (0.5 mg/ml) and Warburgia salutaris aqueous (0.5 mg/mL) extracts. Chemical profiling of the active extracts using gas chromatography-mass spectrometry (GC-MS) identified neophytadiene, guanosine, squalene, cis megastigma-5,8-diene-4-one and sorbitol as prevalent compounds among the active extracts. Anti-quorum sensing activities of E. natalensis (ethyl acetate), A. ferox (methanol) and W. salutaris (aqueous) extracts ranged from 4.81 - 58.34% with E. natalensis (ethyl-acetate) showing the highest activity. Molecular docking against CviR protein showed selected compounds having high docking scores with sorbitol showing the highest score of -7.04 kcal/mol. Warburgia salutaris aqueous extract exhibited the highest biofilm inhibition (73%) against E. coli. Euclea natalensis, Aloe ferox and Warburgia salutaris compounds act as antagonist of N-acyl homoserine lactone (AHL) signaling, thus may serve as candidates in antipathogenic and antibiofilm phytomedicine development for MDR foot ulcer bacterial pathogens.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.departmentPlant Production and Soil Scienceen_US
dc.description.sponsorshipThe South African National Research Foundation (NRF) Thuthuka Grant and the South African Medical Research Council-Self Initiated Research (SAMRC-SIR).en_US
dc.description.urihttps://www.cell.com/heliyon/homeen_US
dc.identifier.citationMashamba, T.G., Adeosun, I.J., Baloyi, I.T., Tshikalange, E.T. & Cosa S. Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants. Heliyon. 2022 Apr 27;8(4):e09303. doi:10.1016/j.heliyon.2022.e09303.en_US
dc.identifier.issn2405-8440 (online)
dc.identifier.other10.1016/j.heliyon.2022.e09303
dc.identifier.urihttps://repository.up.ac.za/handle/2263/88235
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2022 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.subjectAloe feroxen_US
dc.subjectAntiquorum sensingen_US
dc.subjectBiofilm inhibitionen_US
dc.subjectEuclea natalensisen_US
dc.subjectMolecular modellingen_US
dc.subjectMulti-drug resistant pathogensen_US
dc.subjectMultidrug-resistant (MDR)en_US
dc.titleQuorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plantsen_US
dc.typeArticleen_US

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