Comparison of the performance of Chlorococcum ellipsoideum and Tetradesmus obliquus as a carbon source for reduction of Cr(VI) with bacteria

dc.contributor.authorRoestorff, Maria M.
dc.contributor.authorChirwa, Evans M.N.
dc.date.accessioned2019-05-14T09:57:59Z
dc.date.available2019-05-14T09:57:59Z
dc.date.issued2018
dc.description.abstractChromium (Cr) is used in a variety of metallurgical, refractory and chemical processes, as well as in industrial activities such as the refining of ore, the production of steel and alloys, metal plating, and tanneries. Due to its widespread use in anthropogenic processes Cr(VI) is commonly released into the environment. Cr(VI) is known to be carcinogenic and mutagenic to living organisms, however some bacteria species have evolved a detoxification mechanism through which they reduce Cr(VI) to Cr(III), which is 100 times less toxic than Cr(VI). In previous studies of the bioreduction of Cr(VI), glucose and Luria-Bertani broth was used as the primary carbon source. In this study, an indigenous mixed culture of bacteria (Escherichia coli, Bacillus thermoamylovorans and Citrobacter sedlakii) is utilised to reduce Cr(VI) while consuming carbon sources produced by various algae species (Chlorococcum ellipsoideum and Tetradesmus obliquus). The different algae species were compared as possible carbon sources for the bioreduction process. Batch studies show that locally isolated bacteria cocultured with algae achieved 100 % removal of Cr(VI) within 24 h. The performance of the different algae was very similar however bacteria utilizing Tetradesmus obliquus algae as a carbon source achieved the 100 % Cr(VI) reduction the fastest. Algae is sensitive to Cr(VI) toxicity and the algae growth is inhibited, therefore the algae must be cultured beforehand. SEM results indicate that the algae cells were adversely affected by the Cr(VI). The Cr(VI) destroyed the algae cell walls, allowing the bacteria to utilise the internal metabolites. Utilizing carbon sources produced by algae would be more practical to implement in the real world than adding glucose. This study demonstrates the potential of combining locally isolated Cr(VI) reducing bacteria and green algae to decontaminate Cr(VI) polluted sites in South Africa.en_ZA
dc.description.departmentChemical Engineeringen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipThis work is based on the research supported by in part by the National Research Foundation of South Africa.en_ZA
dc.description.urihttp://www.aidic.it/ceten_ZA
dc.identifier.citationRoestorff M.M., Chirwa E.M.N., 2018, Comparison of the performance of Chlorococcum ellipsoideum and Tetradesmus obliquus as a carbon source for reduction of Cr(VI) with bacteria, Chemical Engineering Transactions, 70, 463-468 DOI:10.3303/CET1870078.en_ZA
dc.identifier.issn2283-9216 (online)
dc.identifier.other10.3303/CET1870078
dc.identifier.urihttp://hdl.handle.net/2263/69122
dc.language.isoenen_ZA
dc.publisherThe Italian Association of Chemical Engineeringen_ZA
dc.rights© 2018, AIDIC Servizi S.r.l.en_ZA
dc.subjectAlgaeen_ZA
dc.subjectBacteriologyen_ZA
dc.subjectCarbonen_ZA
dc.subjectDetoxificationen_ZA
dc.subjectEscherichia colien_ZA
dc.subjectGlucoseen_ZA
dc.subjectMetal refiningen_ZA
dc.subjectPlatingen_ZA
dc.subjectRefractory alloysen_ZA
dc.subjectRefractory metalsen_ZA
dc.subjectAnthropogenic processen_ZA
dc.subjectChemical processen_ZA
dc.subjectDetoxification mechanismen_ZA
dc.subjectIndustrial activitiesen_ZA
dc.subjectInternal metabolitesen_ZA
dc.subjectLiving organismsen_ZA
dc.subjectLuria-Bertani brothsen_ZA
dc.subjectPrimary carbonen_ZA
dc.subjectChromium compoundsen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.titleComparison of the performance of Chlorococcum ellipsoideum and Tetradesmus obliquus as a carbon source for reduction of Cr(VI) with bacteriaen_ZA
dc.typeArticleen_ZA

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