Evaluation of traditionally used medicinal plants for anticancer, antioxidant, anti-inflammatory and anti-viral (HPV-1) activity
| dc.contributor.author | Twilley, Danielle | |
| dc.contributor.author | Langhansova, L. | |
| dc.contributor.author | Palaniswamy, D. | |
| dc.contributor.author | Lall, Namrita | |
| dc.contributor.email | namrita.lall@up.ac.za | en_ZA |
| dc.date.accessioned | 2017-06-26T12:34:31Z | |
| dc.date.issued | 2017-09 | |
| dc.description.abstract | The aim of this study was to determine the anticancer, antioxidant, anti-inflammatory and antiviral activities of traditionally used medicinal plants.The extracts were tested for cytotoxicity against human melanoma (A375), epidermoid carcinoma (A431), cervical epithelial carcinoma (HeLa) and human embryonic kidney cells (HEK-293). The antioxidant and anti-inflammatory activities were also determined. Gomphocarpus fruticosus, Helichrysum kraussii and Syzygium jambos were selected for activity against the herpes simplex virus type-1.The extracts exhibited low toxicity towards HEK-293 cells, and four extracts; namely Acacia mellifera, G. fruticosus, H. kraussii and S. jambos, were able to inhibit the A431 and HeLa cells with 50% inhibitory concentrations (IC50) ranging from 34.90-56.20μg/ml. Arbutus unedo, Combretum molle, Dissotis princeps, Erythrophleum lasianthum, Harpephyllum caffrum, H. kraussii and S. jambos, showed high DPPH inhibitory activity, with IC50 values ranging from 2.41-5.25μg/ml. The highest antioxidant activity was seen for S. jambos (DPPH) and A. unedo (NO) respectively with greater activity than ascorbic acid. D. princeps, H. caffrum, Leucas martinicensis and S. jambos, showed high inhibition of the cyclooxygenase-2 (COX-2) enzyme with IC50 values ranging from 3.79-25.80μg/ml with S. jambos showing the highest activity. S. jambos further showed the highest anti-HSV-1 activity at 50.00μg/ml against 100TCID50virus challenge dose.This is the first report of the selected plants for their cytotoxicity, anti-inflammatory and viral inhibitory activity. S. jambos was able to show high inhibition of the HPV type-1 virus and the COX-2 enzyme. | en_ZA |
| dc.description.department | Plant Production and Soil Science | en_ZA |
| dc.description.embargo | 2018-09-30 | |
| dc.description.librarian | hj2017 | en_ZA |
| dc.description.sponsorship | The National Research Foundation of South Africa (84870), the University of Pretoria and the Department of Science and Technology. Walter Sisulu National Botanical Gardens (SANBI). | en_ZA |
| dc.description.uri | http://www.elsevier.com/locate/sajb | en_ZA |
| dc.identifier.citation | Twilley, D., Langhansova, L., Palaniswamy, D. & Lall, N. Evaluation of traditionally used medicinal plants for anticancer, antioxidant, anti-inflammatory and anti-viral (HPV-1) activity, South African Journal of Botany (2017) vol. 112, pp. 494-500, http://dx.doi.org/10.1016/j.sajb.2017.05.021. | en_ZA |
| dc.identifier.issn | 1727-9321 (online) | |
| dc.identifier.issn | 0254-6299 (print) | |
| dc.identifier.other | 10.1016/j.sajb.2017.05.021 | |
| dc.identifier.uri | http://hdl.handle.net/2263/61091 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.rights | © 2017 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 112, pp. 494-500, 2017. doi : 10.1016/j.sajb.2017.05.021. | en_ZA |
| dc.subject | Anticancer | en_ZA |
| dc.subject | Nitric oxide | en_ZA |
| dc.subject | Herpes simplex virus | en_ZA |
| dc.subject | Syzygium jambos | en_ZA |
| dc.subject | Cyclooxygenase-2 (COX-2) | en_ZA |
| dc.subject | 2-diphenyl-1-picryl hydrazyl (DPPH) | en_ZA |
| dc.title | Evaluation of traditionally used medicinal plants for anticancer, antioxidant, anti-inflammatory and anti-viral (HPV-1) activity | en_ZA |
| dc.type | Postprint Article | en_ZA |