Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe

dc.contributor.authorSungirai, Marvelous
dc.contributor.authorBaron, Samantha
dc.contributor.authorMoyo, Doreen Zandile
dc.contributor.authorDe Clercq, Patrick
dc.contributor.authorMaritz-Olivier, Christine
dc.contributor.authorMadder, Maxime
dc.date.accessioned2017-11-28T08:13:30Z
dc.date.issued2018-01
dc.description.abstractAcaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management system in Zimbabwe, screening was done by genotyping Rhipicephalus microplus tick populations using molecular markers associated with resistance to these chemicals. The frequency of the mutant allele for the octopamine/tyramine receptor marker associated with amitraz resistance was high (0.55) and a large proportion 78.5% (288/367) of heterozygote genotypes were observed indicating balancing selection. Of the communal dipping tanks where R. microplus occurred 37.8% (39/103) showed complete resistance genotypes for amitraz. The carboxylesterase marker that has been associated with resistance to organophosphate and pyrethroids indicated no selection pressure in these chemical groups with a low frequency (0.052) of the mutant allele and 89.6% (329/367) sampled ticks showing homozygous susceptibility genotypes. Heterozygous genotypes were present at 27.2% (28/103) of the dipping tanks. The L641 mutation in the voltage–gated sodium channel gene associated with pyrethroid resistance was not detected in Zimbabwean samples. This would suggest a different mechanism of resistance to pyrethroids in these tick populations. Sequence analysis of the octopamine/tyramine receptor gene revealed the presence of other mutations in this region, it will be important to investigate their association with amitraz resistance. These results present the first molecular genotyping of resistance profiles of R. microplus tick populations from Zimbabwe.en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.embargo2019-01-01
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipThe Belgium Department of Development Co-operation (DGD)en_ZA
dc.description.urihttp://www.elsevier.com/locate/ttbdisen_ZA
dc.identifier.citationSungirai, M., Baron, S., Moyo, D.Z., De Clercq, P., Maritz-Olivier, C. & Madder, M. 2018, 'Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe', Ticks and Tick-borne Diseases, vol. 9, no. 1, pp. 2-9.en_ZA
dc.identifier.issn1877-959X ( print)
dc.identifier.issn1877-9603 (online)
dc.identifier.other10.1016/j.ttbdis.2017.10.017
dc.identifier.urihttp://hdl.handle.net/2263/63371
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 9, no. 1, pp. 2-9, 2018. doi : 10.1016/j.ttbdis.2017.10.017.en_ZA
dc.subjectRhipicephalus microplusen_ZA
dc.subjectGenotypingen_ZA
dc.subjectAcaricide resistanceen_ZA
dc.subjectAmitrazen_ZA
dc.subjectZimbabween_ZA
dc.subjectSusceptibilityen_ZA
dc.subjectGene sequenceen_ZA
dc.subjectGene mutationen_ZA
dc.subjectGene frequencyen_ZA
dc.subjectDrug resistanceen_ZA
dc.subjectBalancing selectionen_ZA
dc.subjectCarboxylesterase markeren_ZA
dc.subjectOctopamine/tyramine receptoren_ZA
dc.subjectVoltage-gated sodium channelen_ZA
dc.subject.otherVeterinary science articles SDG-01en_ZA
dc.subject.otherSDG-01: No poverty
dc.titleGenotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabween_ZA
dc.typePostprint Articleen_ZA

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