A systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, Rhipicephalus

dc.contributor.authorMaritz-Olivier, Christine
dc.contributor.authorVan Zyl, W.A. (Willem Andries)
dc.contributor.authorStutzer, Christian
dc.contributor.emailchristine.maritz@up.ac.za.en_US
dc.date.accessioned2013-07-08T08:01:13Z
dc.date.available2013-07-08T08:01:13Z
dc.date.issued2012-06
dc.description.abstractIn the post-genomic era, reverse vaccinology is proving promising in the development of vaccines against bacterial and viral diseases, with limited application in ectoparasite vaccine design. In this study, we present a systematic approach using a combination of functional genomics (DNA microarrays) techniques and a pipeline incorporating in silico prediction of subcellular localization and protective antigenicity using VaxiJen for the identification of novel anti-tick vaccine candidates. A total of 791 candidates were identified using this approach, of which 176 are membrane-associated and 86 secreted soluble proteins. A preliminary analysis on the antigenicity of selected membrane proteins using anti-gut antisera yielded candidates with an IgG binding capacity greater than previously identified epitopes of Bm86. Subsequent vaccination trials using recombinant proteins will not only validate this approach, but will also improve subsequent reverse vaccinology approaches for the identification of novel anti-tick vaccine candidates.en_US
dc.description.librarianhb2013en_US
dc.description.sponsorshipThe Red Meat Research Development Trust, University of Pretoria Research Development Programme, and the Technology and Human Resources for Industry Programme.en_US
dc.description.urihttp://www.elsevier.de/ttbdisen_US
dc.identifier.citationMaritz-Olivier, C, Van Zyl, W & Stutzer, C 2013, 'A systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, Rhipicephalus', Ticks and Tick-borne Diseases, vol. 3, no. 3, pp. 179-187.en_US
dc.identifier.issn1877-959X (print)
dc.identifier.issn1877-9603 (online)
dc.identifier.other10.1016/j.ttbdis.2012.01.003
dc.identifier.urihttp://hdl.handle.net/2263/21874
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2012 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 3, no. 3, 2013. DOI : 10.1016/j.ttbdis.2012.01.003en_US
dc.subjectRhipicephalus microplusen_US
dc.subjectDNA microarrayen_US
dc.subjectCattle ticken_US
dc.subjectReverse vaccinologyen_US
dc.subjectVaccine discoveryen_US
dc.titleA systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, Rhipicephalusen_US
dc.typePostprint Articleen_US

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