New amniotic membrane based biocomposite for future application in reconstructive urology

dc.contributor.authorAdamowicz, Jan
dc.contributor.authorPokrywczyńska, Marta
dc.contributor.authorTworkiewicz, Jakub
dc.contributor.authorKowalczyk, Tomasz
dc.contributor.authorVan Breda, Shane Vontelin
dc.contributor.authorTyloch, Dominik
dc.contributor.authorKloskowski, Tomasz
dc.contributor.authorBodnar, Magda
dc.contributor.authorSkopinska-Wisniewska, Joanna
dc.contributor.authorMarszałek, Andrzej
dc.contributor.authorFrontczak-Baniewicz, Malgorzata
dc.contributor.authorKowalewski, Tomasz A.
dc.contributor.authorDrewa, Tomasz
dc.date.accessioned2016-05-11T10:50:31Z
dc.date.available2016-05-11T10:50:31Z
dc.date.issued2016-01-14
dc.description.abstractOBJECTIVE Due to the capacity of the amniotic membrane (Am) to support re-epithelisation and inhibit scar formation, Am has a potential to become a considerable asset for reconstructive urology i.e., reconstruction of ureters and urethrae. The application of Am in reconstructive urology is limited due to a poor mechanical characteristic. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance, without affecting its unique bioactivity profile. This study evaluated biocomposite material composed of Am and nanofibers as a graft for urinary bladder augmentation in a rat model. MATERIAL AND METHODS Sandwich-structured biocomposite material was constructed from frozen Am and covered on both sides with two-layered membranes prepared from electrospun poly-(L-lactide-co-Ecaprolactone) (PLCL). Wistar rats underwent hemicystectomy and bladder augmentation with the biocomposite material. RESULTS Immunohistohemical analysis (hematoxylin and eosin [H&E], anti-smoothelin and Masson’s trichrome staining [TRI]) revealed effective regeneration of the urothelial and smooth muscle layers. Anti-smoothelin staining confirmed the presence of contractile smooth muscle within a new bladder wall. Sandwich-structured biocomposite graft material was designed to regenerate the urinary bladder wall, fulfilling the requirements for normal bladder tension, contraction, elasticity and compliance. Mechanical evaluation of regenerated bladder wall conducted based on Young’s elastic modulus reflected changes in the histological remodeling of the augmented part of the bladder. The structure of the biocomposite material made it possible to deliver an intact Am to the area for regeneration. An unmodified Am surface supported regeneration of the urinary bladder wall and the PLCL membranes did not disturb the regeneration process. CONCLUSIONS Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance without affecting its unique bioactivity profile.en_ZA
dc.description.departmentInternal Medicineen_ZA
dc.description.librarianam2016en_ZA
dc.description.urihttp://www.plosone.orgen_ZA
dc.identifier.citationAdamowicz J, Pokrywczyńska M, Tworkiewicz J, Kowalczyk T, van Breda SV, Tyloch D, et al. (2016) New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology. PLoS ONE 11(1): e0146012. DOI: 10.1371/journal.pone.0146012.en_ZA
dc.identifier.issn1932-6203
dc.identifier.other10.1371/journal.pone.0146012
dc.identifier.urihttp://hdl.handle.net/2263/52556
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights© 2016 Adamowicz et al. This is an open access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.subjectAmniotic membrane (Am)en_ZA
dc.subjectReconstructionen_ZA
dc.subjectUrinary bladderen_ZA
dc.subjectRat modelen_ZA
dc.titleNew amniotic membrane based biocomposite for future application in reconstructive urologyen_ZA
dc.typeArticleen_ZA

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