Genome and metabolome ms-based mining of a marine strain of Aspergillus affinis

dc.contributor.authorGonçalves, Micael F. M.
dc.contributor.authorHilario, Sandra
dc.contributor.authorTacao, Marta
dc.contributor.authorVan de Peer, Yves
dc.contributor.authorAlves, Artur
dc.contributor.authorEsteves, Ana C.
dc.date.accessioned2022-08-30T11:10:24Z
dc.date.available2022-08-30T11:10:24Z
dc.date.issued2021-12
dc.descriptionSUPPLEMENTARY MATERIAL : Table S1: Gene annotation, Table S2: Carbohydrate active enzymes prediction, Table S3: Secreted proteins, Table S4: Transporter’s prediction, Table S5: Biosynthetic Gene Clusters, Table S6: Summary of genomic features of Circumdati genomes, Table S7: Comparison of CAZymes families between A. affinis CMG 70 and ATCC MYA-4773, Table S8: Full list of compounds, Table S9: List of the significantly differential compounds, File S1: matched spectral library compounds.en_US
dc.description.abstractAspergillus section Circumdati encompasses several species that express both beneficial (e.g., biochemical transformation of steroids and alkaloids, enzymes and metabolites) and harmful compounds (e.g., production of ochratoxin A (OTA)). Given their relevance, it is important to analyze the genetic and metabolic diversity of the species of this section. We sequenced the genome of Aspergillus affinis CMG 70, isolated from sea water, and compared it with the genomes of species from section Circumdati, including A. affinis’s strain type. The A. affinis genome was characterized considering secondary metabolites biosynthetic gene clusters (BGCs), carbohydrate-active enzymes (CAZymes), and transporters. To uncover the biosynthetic potential of A. affinis CMG 70, an untargeted metabolomics (LC-MS/MS) approach was used. Cultivating the fungus in the presence and absence of sea salt showed that A. affinis CMG 70 metabolite profiles are salt dependent. Analyses of the methanolic crude extract revealed the presence of both unknown and well-known Aspergillus compounds, such as ochratoxin A, anti-viral (e.g., 3,5-Di-tert-butyl-4-hydroxybenzoic acid and epigallocatechin), anti-bacterial (e.g., 3-Hydroxybenzyl alcohol, L-pyroglutamic acid, lecanoric acid), antifungal (e.g., L-pyroglutamic acid, 9,12,13-Trihydroxyoctadec-10-enoic acid, hydroxyferulic acid), and chemotherapeutic (e.g., daunomycinone, mitoxantrone) related metabolites. Comparative analysis of 17 genomes from 16 Aspergillus species revealed abundant CAZymes (568 per species), secondary metabolite BGCs (73 per species), and transporters (1359 per species). Some BGCs are highly conserved in this section (e.g., pyranonigrin E and UNII-YC2Q1O94PT (ACR toxin I)), while others are incomplete or completely lost among species (e.g., bikaverin and chaetoglobosins were found exclusively in series Sclerotiorum, while asperlactone seemed completely lost). The results of this study, including genome analysis and metabolome characterization, emphasize the molecular diversity of A. affinis CMG 70, as well as of other species in the section Circumdati.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2022en_US
dc.description.sponsorshipThe authors acknowledge financial support from the Portuguese Foundation for Science and Technology (FCT) to CESAM (UIDB/50017/2020+UIDP/50017/2020), Marta Tacão (CEECIND/00977/2020) and the PhD grants of M. Gonçalves (SFRH/BD/129020/2017) and S. Hilário (SFRH/BD/137394/2018).en_US
dc.description.urihttps://www.mdpi.com/journal/jofen_US
dc.identifier.citationGonçalves, M.F.M.; Hilário, S.; Tacão, M.; Van de Peer, Y.; Alves, A.; Esteves, A.C. Genome and Metabolome MS-Based Mining of a Marine Strain of Aspergillus affinis. Journal of Fungi 2021, 7, 1091. https://DOI.org/10.3390/jof7121091.en_US
dc.identifier.issn2309-608X
dc.identifier.issn10.3390/jof7121091
dc.identifier.urihttps://repository.up.ac.za/handle/2263/86999
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectAntimicrobialen_US
dc.subjectAnti-canceren_US
dc.subjectComparative genomicsen_US
dc.subjectMarine fungien_US
dc.subjectMetabolitesen_US
dc.subjectWhole genome sequencing (WGS)en_US
dc.titleGenome and metabolome ms-based mining of a marine strain of Aspergillus affinisen_US
dc.typeArticleen_US

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