Enterococcus spp. cell-free extract : an abiotic route for synthesis of selenium nanoparticles (SeNPs), their characterisation and inhibition of Escherichia coli

dc.contributor.authorTendenedzai, Job Tatenda
dc.contributor.authorChirwa, Evans M.N.
dc.contributor.authorBrink, Hendrik Gideon
dc.contributor.emaildeon.brink@up.ac.zaen_US
dc.date.accessioned2023-09-26T13:07:17Z
dc.date.available2023-09-26T13:07:17Z
dc.date.issued2022-02-16
dc.descriptionDATA AVAILABILITY STATEMENT : The data presented in this study are openly available in the University of Pretoria Research Data Repository at doi:10.25403/UPresearchdata.19169507 (accessed on 1 February 2022).en_US
dc.description.abstractSelenite (SeO3 2), the most toxic and most reactive selenium (Se) oxyanion, can be reduced to elemental selenium (Se0) nanoparticles by a variety of bacteria, including Enterococcus spp. Previously, the orthodox view held that the reduction of SeO3 2 to Se0 by a wide range of bacteria was solely accomplished by biological processes; however, recent studies have shown that various bacterial strains secrete metal-reducing metabolites, thereby indirectly catalysing the reduction of these metal species. In the current study, selenium nanoparticles were synthesised from the abiotic reduction of selenite with the use of Enterococcus spp. cell-free extract. Once separated from the cellfree extract, the particles were analysed using Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM) and a Zetasizer. The results revealed that the SeNPs were spherical in shape, containing both amorphous and crystalline properties, and the sizes with the highest frequency ranged close to 200 nm. Additionally, the obtained nanoparticles exhibited antimicrobial properties by directly inhibiting the viability of an E. coli bacterial strain. The results demonstrate not only the potential of abiotic production of SeNPs, but also the potential for these particles as microbial inhibitors in medical or similar fields.en_US
dc.description.departmentChemical Engineeringen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe National Research Foundation of South Africa.en_US
dc.description.urihttps://www.mdpi.com/journal/nanomaterialsen_US
dc.identifier.citationTendenedzai, J.T.; Chirwa, E.M.N.; Brink, H.G. Enterococcus spp. Cell-Free Extract: An Abiotic Route for Synthesis of Selenium Nanoparticles (SeNPs), Their Characterisation and Inhibition of Escherichia coli. Nanomaterials 2022, 12, 658. https://DOI.org/10.3390/nano12040658.en_US
dc.identifier.issn2079-4991
dc.identifier.other10.3390/nano12040658
dc.identifier.urihttp://hdl.handle.net/2263/92416
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectSeleniteen_US
dc.subjectAbioticen_US
dc.subjectCell-free extracten_US
dc.subjectSelenium nanoparticlesen_US
dc.subjectAntibacterialen_US
dc.subjectFourier transform infrared spectroscopy (FTIR)en_US
dc.subjectZetasizeren_US
dc.subjectX-ray diffraction (XRD)en_US
dc.subjectTransmission electron microscopy (TEM)en_US
dc.titleEnterococcus spp. cell-free extract : an abiotic route for synthesis of selenium nanoparticles (SeNPs), their characterisation and inhibition of Escherichia colien_US
dc.typeArticleen_US

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