Diversity of Bacillus cereus strains in extended shelf life

dc.contributor.authorMugadza, D.T. (Desmond)
dc.contributor.authorBuys, E.M. (Elna Maria)
dc.contributor.emailelna.buys@up.ac.zaen_ZA
dc.date.accessioned2018-01-17T08:16:54Z
dc.date.issued2017-10
dc.description.abstractCharacterisation of 49 Bacillus cereus strains obtained from extended shelf life (ESL) milk and filler nozzles was done using (GTG)5 rep PCR fingerprinting, determining the presence of the virulence genes cytK, nheA, cer and hblA, and discrimination of psychrotrophic and mesophilic strains with 16S rDNA. Fourteen isolates were selected for 16S partial sequencing. Fingerprinting and sequencing showed evidence of filler nozzles contaminating ESL milk despite high heterogeneity existing between the isolates. While there is high prevalence of cer, hblA and nheA; cytK was not widely distributed. There was 100% and 8% prevalence of mesophilic and psychrotrophic signatures, respectively. Despite the large diversity of the B. cereus strains in this study, there is evidence that filler nozzles and raw milk are a source of contamination of B. cereus in ESL milk.en_ZA
dc.description.departmentFood Scienceen_ZA
dc.description.embargo2018-10-30
dc.description.librarianhj2018en_ZA
dc.description.urihttp://www.elsevier.com/locate/idairyjen_ZA
dc.identifier.citationMugadza, D.T. & Buys, E.M. 2017, 'Diversity of Bacillus cereus strains in extended shelf life', International Dairy Journal, vol. 73, no. 144-150.en_ZA
dc.identifier.issn0958-6946 (print)
dc.identifier.issn1879-0143 (online)
dc.identifier.other10.1016/j.idairyj.2017.06.001
dc.identifier.urihttp://hdl.handle.net/2263/63578
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in International Dairy Journal . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in International Dairy Journal, vol. 73, pp. 144-150, 2017. doi : 10.1016/j.idairyj.2017.06.001.en_ZA
dc.subjectExtended shelf life (ESL)en_ZA
dc.subjectBacteriaen_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectGenesen_ZA
dc.subjectContaminationen_ZA
dc.subjectDairy planten_ZA
dc.subjectMesophilic strainsen_ZA
dc.subjectRaw milken_ZA
dc.subjectEmetic toxinen_ZA
dc.subjectPasteurized milken_ZA
dc.subjectFood poisoning toxinsen_ZA
dc.subjectEnterotoxin haemolysin BL (hblA)en_ZA
dc.subjectEmetic toxin cereulide (cer)en_ZA
dc.subjectNonhemolytic enterotoxin A (nheA)en_ZA
dc.subjectCytotoxin K (cytK)en_ZA
dc.subjectBacillus cereusen_ZA
dc.subjectMilken_ZA
dc.titleDiversity of Bacillus cereus strains in extended shelf lifeen_ZA
dc.typePostprint Articleen_ZA

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