Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)

dc.contributor.authorDubey, Jitender P.
dc.contributor.authorHilali, M.
dc.contributor.authorVan Wilpe, Erna
dc.contributor.authorVerma, Shiv Kumar
dc.contributor.authorCalero-Bernal, Rafael
dc.contributor.authorAbdel-Wahab, A.
dc.date.accessioned2014-08-25T11:13:07Z
dc.date.issued2015-02
dc.description.abstractFour valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 μm thick, depending on the section and the technique. In 5 μm paraffin-embedded sections, the sarcocyst wall was indistinct, 2–5 μm thick and appeared smooth. In 1 μm plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2·5–5·2 μm thick and had branched villar Protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the arcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 μm long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11·2 to 16·8 μm in length. By TEM, bradyzoites had a very long (10 μm) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipR. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund.en_US
dc.description.urihttp://journals.cambridge.org/action/displayJournal?jid=PARen_US
dc.identifier.citationDubey, JP, Hilali, M, Van Wilpe, E, Verma, SK, Calero-Bernal, R & Abdel-Wahab, A 2014, 'Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)', Parasitology, vol. 142, no. 2, pp. 385-394.en_US
dc.identifier.issn0031-1820 (print)
dc.identifier.issn1469-8161 (online)
dc.identifier.other10.1017/S003118201400122X
dc.identifier.urihttp://hdl.handle.net/2263/41582
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.rights© Cambridge University Press 2014.en_US
dc.subjectSarcocystis fusiformisen_US
dc.subjectSarcocysten_US
dc.subjectWater buffalo (Bubalus bubalis)en_US
dc.subjectElectron microscopyen_US
dc.subjectUltrastructureen_US
dc.subjectBubalus bubalis
dc.titleRedescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)en_US
dc.typePostprint Articleen_US

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