Rhizosphere diazotrophs and other bacteria associated with native and encroaching legumes in the succulent Karoo biome in South Africa

dc.contributor.authorMuema, Esther K.
dc.contributor.authorSteenkamp, Emma Theodora
dc.contributor.authorVenter, S.N. (Stephanus Nicolaas)
dc.contributor.emailesther.muema@fabi.up.ac.zaen_US
dc.date.accessioned2023-09-21T08:04:30Z
dc.date.available2023-09-21T08:04:30Z
dc.date.issued2022-01-20
dc.descriptionSUPPLEMENTARY MATERIAL : TABLE S1: Statistical analysis of soil chemical properties of the study sites showing main effects of different legume species and sites, TABLE S2: Diverse bacterial phyla in the Succulent Karoo biome in South Africa, their inherent properties and possible impacts on plant communities, TABLE S3: Families containing diazotrophic bacteria (nifH gene) in the rhizosphere soils examined and their possible roles in plant growth promotion of legume species in the Succulent Karoo biome in South Africa, TABLE S4: Analysis of variance (ANOVA) and Permutations analysis of variance (PERMANOVA) to test the effects of factors ‘legume species’, ‘sites’ and their interactions on the respective alpha and beta diversity matrices, using the 16S rRNA and nifH gene barcodes.en_US
dc.description.abstractTotal and diazotrophic bacteria were assessed in the rhizosphere soils of native and encroaching legumes growing in the Succulent Karoo Biome (SKB), South Africa. These were Calobota sericea, Lessertia diffusa, Vachellia karroo, and Wiborgia monoptera, of Fabaceae family near Springbok (Northern Cape Province) and neighboring refugia of the Fynbos biome for C. sericea for comparison purposes. Metabarcoding approach using 16S rRNA gene revealed Actinobacteria (26.7%), Proteobacteria (23.6%), Planctomycetes, and Acidobacteria (10%), while the nifH gene revealed Proteobacteria (70.3%) and Cyanobacteria (29.5%) of the total sequences recovered as the dominant phyla. Some of the diazotrophs measured were assigned to families; Phyllobacteriaceae (39%) and Nostocaceae (24.4%) (all legumes), Rhodospirillaceae (7.9%), Bradyrhizobiaceae (4.6%) and Methylobacteriaceae (3%) (C. sericea, V. karroo, W. monoptera), Rhizobiaceae (4.2%; C. sericea, L. diffusa, V. Karroo), Microchaetaceae (4%; W. monoptera, V. karroo), Scytonemataceae (3.1%; L. diffusa, W. monoptera), and Pseudomonadaceae (2.7%; V. karroo) of the total sequences recovered. These families have the potential to fix the atmospheric nitrogen. While some diazotrophs were specific or shared across several legumes, a member of Mesorhizobium species was common in all rhizosphere soils considered. V. karroo had statistically significantly higher Alpha and distinct Beta-diversity values, than other legumes, supporting its influence on soil microbes. Overall, this work showed diverse bacteria that support plant life in harsh environments such as the SKB, and shows how they are influenced by legumes.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipDSI-NRF Centre of Excellence in Plant Health and Biotechnology (CPHB) through the Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa.en_US
dc.description.urihttps://www.mdpi.com/journal/microorganismsen_US
dc.identifier.citationMuema, E.K.; Steenkamp, E.T.; Venter, S.N. Rhizosphere Diazotrophs and Other Bacteria Associated with Native and Encroaching Legumes in the Succulent Karoo Biome in South Africa. Microorganisms 2022, 10, 216. https://DOI.org/10.3390/microorganisms10020216.en_US
dc.identifier.issn2076-2607 (online)
dc.identifier.other10.3390/microorganisms10020216
dc.identifier.urihttp://hdl.handle.net/2263/92365
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectLegumes speciesen_US
dc.subjectDiazotrophsen_US
dc.subjectMetabarcodingen_US
dc.subjectRhizosphere soilsen_US
dc.subject16S rRNA Geneen_US
dc.subjectnifH Geneen_US
dc.subjectSucculent Karoo Biome (SKB)en_US
dc.subjectSouth Africa (SA)en_US
dc.titleRhizosphere diazotrophs and other bacteria associated with native and encroaching legumes in the succulent Karoo biome in South Africaen_US
dc.typeArticleen_US

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