Hypoglycemic evaluation of a new triterpene and other compounds isolated from Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark

dc.contributor.authorDeutschlander, M.S. (Miranda Susan)
dc.contributor.authorLall, Namrita
dc.contributor.authorVan de Venter, Maryna
dc.contributor.authorHussein, Ahmed A.
dc.contributor.emailmiranda.deutschlander@up.ac.zaen_US
dc.date.accessioned2011-04-28T07:08:26Z
dc.date.available2011-04-28T07:08:26Z
dc.date.issued2011-02
dc.description.abstractAIM OF THE STUDY: Investigate the hypoglycaemic activity of the four isolated compounds from a crude acetone extract of the root bark of Euclea undulata var. myrtina, which is used by traditional healers in the Venda area, Limpopo Province in the treatment of diabetes. MATERIAL AND METHODS: The hypoglycaemic activity of the four compounds isolated from Euclea undulata was determined by in vitro screening of glucose utilization by C2C12 myocytes at a concentration of 25 g/ml or 50 g/ml. The inhibition of -glucosidase was also tested at concentrations ranging from 0.02 to 200.00 g/ml. RESULTS: Assay-guided isolation of the crude acetone extract of the root bark of Euclea undulata var. myrtina afforded a new triterpene, -amyrin-3O- -(5-hydroxy) ferulic acid (1), in addition to three known compounds; betulin (2), lupeol (3) and epicatechin (4). The in vitro results on C2C12 myocytes suggest that compound 4 may have some effect to lowers blood glucose levels, whereas compound 1 has the ability to inhibit -glucosidase at a concentration of 200.0 g/ml with an IC50 value of 4.79 that correlates with that of the positive control acarbose IC50 value 4.75. CONCLUSION: The results suggest that 4 may have some ability to lower blood glucose levels, whereas 1 has the ability to inhibit -glucosidase. ETHNOPHARMACOLOGICAL RELEVANCE: These findings corroborate the ethnomedicinal use of Euclea undulata by traditional healers for the treatment of diabetes as two substances was isolated from the acetone plant extract that exhibit hypoglycaemic activity.en
dc.identifier.citationDeutschländer, MS, Lall, N, Van de Venter, M & Hussein, AA 2011, 'Hypoglycemic evaluation of a new triterpene and other compounds isolated from Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark', Journal of Ethnopharmacology, vol. 133, no. 3, pp. 1091-1095. [www.elsevier.com/locate/jethpharm]en
dc.identifier.issn1091-1095
dc.identifier.issn0378-8741 (online)
dc.identifier.other10.1016/j.jep.2010.11.038
dc.identifier.urihttp://hdl.handle.net/2263/16369
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2010 Elsevier Ireland Ltd. All rights reserved.en_US
dc.subjectα-Glucosidaseen
dc.subjectC2C12 myocytesen
dc.subjectEuclea undulataen
dc.subjectHypoglycaemic activityen
dc.subjectNew triterpeneen
dc.subject.lcshEbenaceaeen
dc.subject.lcshHypoglycemiaen
dc.subject.lcshDiabetes -- Treatment -- South Africa -- Limpopoen
dc.subject.lcshTraditional medicine -- South Africa -- Limpopoen
dc.titleHypoglycemic evaluation of a new triterpene and other compounds isolated from Euclea undulata Thunb. var. myrtina (Ebenaceae) root barken
dc.typePostprint Articleen

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