The GC–MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiology

dc.contributor.authorMalatji, Bontle G.
dc.contributor.authorMason, Shayne
dc.contributor.authorMienie, Lodewyk J.
dc.contributor.authorWevers, Ron A.
dc.contributor.authorMeyer, Helgard Pieter
dc.contributor.authorVan Reenen, Mari
dc.contributor.authorReinecke, Carolus J.
dc.date.accessioned2020-06-09T06:56:43Z
dc.date.available2020-06-09T06:56:43Z
dc.date.issued2019-03
dc.description.abstractINTRODUCTION : Fibromyalgia syndrome (FMS) is a chronic pain syndrome. Previous analyses of untargeted metabolomics data indicated altered metabolic profile in FMS patients. OBJECTIVES : We report a semi-targeted explorative metabolomics study on the urinary metabolite profile of FMS patients; exploring the potential of urinary metabolite information to augment existing medical diagnosis. METHODS : All cases were females. Patients had a medical history of persistent FMS (n = 18). Control groups were first-generation family members of the patients (n = 11), age-related individuals without indications of FMS (n = 10), and healthy, young (18–22 years) individuals (n = 41). The biofluid investigated was early morning urine samples. Data generation was done through gas chromatography–mass spectrometry (GC–MS) analysis and data processing and analyses were performed using Matlab, R, SPSS and SAS software. RESULTS : Quantitative analysis revealed the presence of 196 metabolites. Unsupervised and supervised multivariate analyses distinguished all three control groups and the FMS patients, which could be related to 14 significantly increased metabolites. These metabolites are associated with energy metabolism, digestion and metabolism of carbohydrates and other host and gut metabolites. CONCLUSIONS : Overall, urinary metabolite profiles in the FMS patients suggest: (1) energy utilization is a central aspect of this pain disorder, (2) dysbiosis seems to prevail in FMS patients, indicated by disrupted microbiota metabolites, supporting the model that microbiota may alter brain function through the gut-brain axis, with the gut being a gateway to generalized pain, and (3) screening of urine from FMS is an avenue to explore for adding non-invasive clinical information for diagnosis and treatment of FMS.en_ZA
dc.description.departmentFamily Medicineen_ZA
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipThe Technological Innovation Agency (TIA) of the South African Department of Science and Technology (DST) and from the Nuclear Technologies in Medicine and the Biosciences Initiative (NTeMBI) of the Nuclear Energy Corporation of South Africa (NECSA). BM received a postgraduate bursary from the National Research Foundation (NRF) of South Africa.en_ZA
dc.description.urihttp://link.springer.com/journal/11306en_ZA
dc.identifier.citationMalatji, B.G., Mason, S., Mienie, L.J. et al. The GC–MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiology. Metabolomics 15, 54 (2019). https://doi.org/10.1007/s11306-019-1513-6.en_ZA
dc.identifier.issn1573-3882 (print)
dc.identifier.issn1573-3890 (online)
dc.identifier.other10.1007/s11306-019-1513-6
dc.identifier.urihttp://hdl.handle.net/2263/74906
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media, LLC, part of Springer Nature 2019. The original publication is available at : http://link.springer.comjournal/11306.en_ZA
dc.subjectFibromyalgia syndrome (FMS)en_ZA
dc.subjectGas chromatography–mass spectrometry (GC–MS)en_ZA
dc.subjectDysbiosisen_ZA
dc.subjectCarbohydrateen_ZA
dc.subjectPainen_ZA
dc.subjectBiomarkersen_ZA
dc.subject.otherHealth sciences articles SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.titleThe GC–MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiologyen_ZA
dc.typePostprint Articleen_ZA

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