Molecular epidemiology of extended-spectrum beta-lactamase–producing extra-intestinal pathogenic Escherichia coli strains over a 2-year period (2017–2019) from Zimbabwe

dc.contributor.authorTakawira, Faustinos Tatenda
dc.contributor.authorPitout, Johann D.D.
dc.contributor.authorThilliez, Gaetan
dc.contributor.authorMashe, Tapfumanei
dc.contributor.authorGutierrez, Ana Victoria
dc.contributor.authorKingsley, Robert A.
dc.contributor.authorPeirano, Gisele
dc.contributor.authorMatheu, Jorge
dc.contributor.authorMidzi, Stanley M.
dc.contributor.authorMwamakamba, Lusubilo W.
dc.contributor.authorGally, David L.
dc.contributor.authorTarupiwa, Andrew
dc.contributor.authorMukavhi, Leckson
dc.contributor.authorEhlers, Marthie Magdaleen
dc.contributor.authorMtapuri-Zinyowera, Sekesai
dc.contributor.authorKock, Marleen M.
dc.contributor.emailmarleen.kock@up.ac.zaen_ZA
dc.date.accessioned2022-02-24T09:31:20Z
dc.date.issued2022
dc.description.abstractThis study was designed to characterize extended-spectrum beta-lactamase (ESBL)–producing extra-intestinal pathogenic Escherichia coli (E.coli) (ExPEC) associated with urinary tract infections in nine different geographic regions of Zimbabwe over a 2-year period (2017–2019). A total of 48 ESBL-positive isolates from urine specimen were selected for whole-genome sequencing from 1246 Escherichia coli isolates biobanked at the National Microbiology Reference laboratory using phenotypic susceptibility testing results from the National Escherichia coli Surveillance Programme to provide representation of different geographical regions and year of isolation. The majority of ESBL E. coli isolates produced cefotaximase-Munich (CTX-M)-15, CTX-M-27, and CTX-M-14. In this study, sequence types (ST) 131 and ST410 were the most predominant antimicrobial-resistant clones and responsible for the increase in ESBL–producing E. coli strains since 2017. Novel ST131 complex strains were recorded during the period 2017 to 2018, thus showing the establishment and evolution of this antimicrobial-resistant ESBL clone in Zimbabwe posing an important public health threat. Incompatibility group F plasmids were predominant among ST131 and ST410 isolates with the following replicons recorded most frequently: F1:A2:B20 (9/19, 47%), F2:A1: B (5/19, 26%), and F1:A1:B49 (8/13, 62%). The results indicate the need for continuous tracking of different ESBL ExPEC clones on a global scale, while targeting specific STs (e.g. ST131 and ST410) through control programs will substantially decrease the spread of ESBLs among ExPEC.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.embargo2022-11-15
dc.description.librarianhj2022en_ZA
dc.description.sponsorshipThe National Health Laboratory Service (NHLS), the University of Pretoria, South Africa, and a strategic partnership between National Microbiology Reference Laboratory and Quadrum Institute Biosciences.en_ZA
dc.description.urihttp://link.springer.com/journal/10096en_ZA
dc.identifier.citationTakawira, F.T., Pitout, J., Thilliez, G. et al. Molecular epidemiology of extended-spectrum beta-lactamase–producing extra-intestinal pathogenic Escherichia coli strains over a 2-year period (2017–2019) from Zimbabwe. European Journal of Clinical Microbiology and Infectious Diseases (2022). https://doi.org/10.1007/s10096-021-04379-z. NYP.en_ZA
dc.identifier.issn0934-9723 (print)
dc.identifier.issn1435-4373 (online)
dc.identifier.other10.1007/s10096-021-04379-z
dc.identifier.urihttp://hdl.handle.net/2263/84181
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. The original publication is available at : http://link.springer.comjournal/10096.en_ZA
dc.subjectExtended-spectrum beta-lactamase (ESBL)en_ZA
dc.subjectEscherichia colien_ZA
dc.subjectST131en_ZA
dc.subjectST410en_ZA
dc.subjectC1-M27en_ZA
dc.subjectZimbabween_ZA
dc.titleMolecular epidemiology of extended-spectrum beta-lactamase–producing extra-intestinal pathogenic Escherichia coli strains over a 2-year period (2017–2019) from Zimbabween_ZA
dc.typePostprint Articleen_ZA

Files

Original bundle

Now showing 1 - 5 of 6
Loading...
Thumbnail Image
Name:
Takawira_Molecular_2022.pdf
Size:
1.52 MB
Format:
Adobe Portable Document Format
Description:
Postprint Article
Loading...
Thumbnail Image
Name:
Takawira_MolecularSupplFile1_2022.pdf.xlsx
Size:
26.35 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 1
Loading...
Thumbnail Image
Name:
Takawira_MolecularSupplFile2_2022.xlsx
Size:
39.55 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 2
Loading...
Thumbnail Image
Name:
Takawira_MolecularSupplFile3_2022.xlsx
Size:
22.97 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 3
Loading...
Thumbnail Image
Name:
Takawira_MolecularSupplFile4_2022.xlsx
Size:
48.49 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 4

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: