The first report of Escherichia fergusonii isolated from non-human primates, in Africa

dc.contributor.authorGlover, Barbara
dc.contributor.authorWentzel, Jeanette Maria
dc.contributor.authorJenkins, Akinbowale Olajide
dc.contributor.authorVan Vuuren, Moritz
dc.date.accessioned2018-08-03T12:08:18Z
dc.date.available2018-08-03T12:08:18Z
dc.date.issued2017-06
dc.description.abstractThe aim of this study was to determine the resistance phenotypes of selected enteric bacteria isolated from nonhuman primates at a wildlife-human interface. Bacterial isolates from faecal samples of non-human primates at two wildlife rehabilitation centres in South Africa were screened for the presence of Escherichia coli. The biochemical characterisation of E. coli and E. coli-like bacteria revealed both adonitol positive and sorbitol negative strains – a unique characteristic of Escherichia fergusonii and Escherichia coli K99. Further tests were carried out to identify the isolates, namely growth on Simmons citrate agar supplemented with 2% adonitol and biochemical tests based on their ability to ferment cellobiose and D-arabitol. Antimicrobial sensitivity was determined with microbroth dilution tests employing microtitre plates with 21 different antimicrobial drugs. Molecular characterisation was done with a duplex polymerase chain reaction (PCR) assay that targeted the yliE and EFER_1569 genes. E. fergusonii strains were confirmed by the presence of a 233 bp segment of the yliE gene and a 432 bp segment of the EFER_1569 gene. Twenty-three E. coli-like bacteria were confirmed as E. fergusonii based on the confirmatory tests and they were in 100% agreement. Approximately 87% of them were resistant to polymyxins B and E (colistin) as well as the carbapenem group with occasional resistance to amikacin. This is the first reported isolation and identification of E. fergusonii strains in non-human primates. The findings point to E. fergusonii as a possible emerging pathogen of zoonotic importance.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2018en_ZA
dc.description.urihttps://www.elsevier.com/locate/onehlten_ZA
dc.identifier.citationGlover, B., Wentzel, J., Jenkins, A.O. & Van Vuuren, M. 2017, 'The first report of Escherichia fergusonii isolated from non-human primates, in Africa', One Health, vol. 3, pp. 70-75.en_ZA
dc.identifier.issn2352-7714 (online)
dc.identifier.other10.1016/j.onehlt.2017.05.001
dc.identifier.urihttp://hdl.handle.net/2263/66092
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).en_ZA
dc.subjectEscherichia fergusoniien_ZA
dc.subjectAntimicrobial resistanceen_ZA
dc.subjectNon-human primatesen_ZA
dc.subjectWildlifeen_ZA
dc.titleThe first report of Escherichia fergusonii isolated from non-human primates, in Africaen_ZA
dc.typeArticleen_ZA

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