The first report of Escherichia fergusonii isolated from non-human primates, in Africa
dc.contributor.author | Glover, Barbara | |
dc.contributor.author | Wentzel, Jeanette Maria | |
dc.contributor.author | Jenkins, Akinbowale Olajide | |
dc.contributor.author | Van Vuuren, Moritz | |
dc.date.accessioned | 2018-08-03T12:08:18Z | |
dc.date.available | 2018-08-03T12:08:18Z | |
dc.date.issued | 2017-06 | |
dc.description.abstract | The aim of this study was to determine the resistance phenotypes of selected enteric bacteria isolated from nonhuman primates at a wildlife-human interface. Bacterial isolates from faecal samples of non-human primates at two wildlife rehabilitation centres in South Africa were screened for the presence of Escherichia coli. The biochemical characterisation of E. coli and E. coli-like bacteria revealed both adonitol positive and sorbitol negative strains – a unique characteristic of Escherichia fergusonii and Escherichia coli K99. Further tests were carried out to identify the isolates, namely growth on Simmons citrate agar supplemented with 2% adonitol and biochemical tests based on their ability to ferment cellobiose and D-arabitol. Antimicrobial sensitivity was determined with microbroth dilution tests employing microtitre plates with 21 different antimicrobial drugs. Molecular characterisation was done with a duplex polymerase chain reaction (PCR) assay that targeted the yliE and EFER_1569 genes. E. fergusonii strains were confirmed by the presence of a 233 bp segment of the yliE gene and a 432 bp segment of the EFER_1569 gene. Twenty-three E. coli-like bacteria were confirmed as E. fergusonii based on the confirmatory tests and they were in 100% agreement. Approximately 87% of them were resistant to polymyxins B and E (colistin) as well as the carbapenem group with occasional resistance to amikacin. This is the first reported isolation and identification of E. fergusonii strains in non-human primates. The findings point to E. fergusonii as a possible emerging pathogen of zoonotic importance. | en_ZA |
dc.description.department | Veterinary Tropical Diseases | en_ZA |
dc.description.librarian | am2018 | en_ZA |
dc.description.uri | https://www.elsevier.com/locate/onehlt | en_ZA |
dc.identifier.citation | Glover, B., Wentzel, J., Jenkins, A.O. & Van Vuuren, M. 2017, 'The first report of Escherichia fergusonii isolated from non-human primates, in Africa', One Health, vol. 3, pp. 70-75. | en_ZA |
dc.identifier.issn | 2352-7714 (online) | |
dc.identifier.other | 10.1016/j.onehlt.2017.05.001 | |
dc.identifier.uri | http://hdl.handle.net/2263/66092 | |
dc.language.iso | en | en_ZA |
dc.publisher | Elsevier | en_ZA |
dc.rights | © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). | en_ZA |
dc.subject | Escherichia fergusonii | en_ZA |
dc.subject | Antimicrobial resistance | en_ZA |
dc.subject | Non-human primates | en_ZA |
dc.subject | Wildlife | en_ZA |
dc.title | The first report of Escherichia fergusonii isolated from non-human primates, in Africa | en_ZA |
dc.type | Article | en_ZA |