Evaluation of two topical treatment methods for the elimination of Taylorella equigenitalis in stallions

dc.contributor.advisorSchulman, Martin L.en
dc.contributor.postgraduateKeys, Bronwyn
dc.date.accessioned2015-07-02T11:08:32Z
dc.date.available2015-07-02T11:08:32Z
dc.date.created2015/04/22en
dc.date.issued2014en
dc.descriptionDissertation (MSc)--University of Pretoria, 2014.en
dc.description.abstractTaylorella equigenitalis, the aetiological agent for contagious equine metritis (CEM) is sensitive to a wide range of antimicrobial drugs in vitro. Current treatments are anectdotal and validated protocols are undefined. The objective of this study was to compare the efficacy of two topical antimicrobial agents and the duration of treatment required to eliminate T. equigenitalis in stallions. The treatment process was strictly monitored with daily quantitative polymerase chain reaction (qPCR) sampling and testing of the colonised stallion to determine an effective treatment duration and to note trends in cycle threshold (Ct) values. The study was approved by the University of Pretoria’s Animal Use and Care Committee (V 068/12) according to the South African National Standard (SANS 10386: 2008) for the care and use of animals for scientific purposes. Twenty three stallions colonised with Taylorella equigenitalis were randomly assigned to either one of two topical treatment categories: 1% silver sulphadiazine (n=11) and 0.2% nitrofurazone (n=12). A standardised treatment protocol consisting of topical cleansing and antimicrobial application was performed. Descriptive statistical analysis of the treatment duration and cycle threshold (Ct) values obtained from qPCR was performed to compare the two antimicrobial agents and to determine trends in the data. This study can provide a validated treatment and monitoring protocol for the topical treatment of CEM in stallions.en
dc.description.availabilityUnrestricteden
dc.description.degreeMScen
dc.description.departmentCompanion Animal Clinical Studiesen
dc.description.librariantm2015en
dc.identifier.citationKeys, B 2014, Evaluation of two topical treatment methods for the elimination of Taylorella equigenitalis in stallions, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/46267>en
dc.identifier.otherA2015en
dc.identifier.urihttp://hdl.handle.net/2263/46267
dc.language.isoenen
dc.publisherUniversity of Pretoriaen_ZA
dc.rights© 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.en
dc.subjectUCTDen
dc.titleEvaluation of two topical treatment methods for the elimination of Taylorella equigenitalis in stallionsen
dc.typeDissertationen

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