Integrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification : a case study on south-east African Erythroxylaceae species

dc.contributor.authorAlberts, P.S.F. (Sewes)
dc.contributor.authorMeyer, Jacobus Johannes Marion
dc.contributor.emailsewes.alberts@up.ac.zaen_ZA
dc.date.accessioned2021-12-01T05:47:47Z
dc.date.issued2022-05
dc.description.abstractPlants have been used as medicines for millennia and are major contributors to developed western pharmacopoeia. The Erythroxylum and Nectaropetalum genera belong to the Erythroxylaceae (coca) family, with select species capable of producing highly valued ‘blockbuster’ medicinal compounds including, amongst others atropine, cocaine, scopolamine, and tigloidine. Erythroxylum delagoense, E. emarginatum, E. pictum, N. capense and N. zuluense are indigenous to the south-east tropical regions of Africa. The morphological similarity between these taxa make identification to species-level troublesome and often unreliable, indicating a need for alternative identification methods. This study aimed to compare gas chromatography-mass spectrometry (GC-MS)- and nuclear magnetic resonance (NMR)-based metabolomics analyses with DNA barcoding to evaluate the identifying characteristics of these coca species. The results emphasise the importance of integrating chemotaxonomy and DNA barcoding techniques in plant identification. In this sample of the Erythroxylaceae, the differentiating identification accuracy was shown to increase from morphology to DNA barcoding to chemotaxonomy. This study further highlights the strengths and weaknesses of various plant identification strategies, as well as providing a developing model for more accurate and reliable species-level identification of plants. The findings from this case study could aid in the identification and classification of other closely related taxa.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.embargo2022-10-29
dc.description.librarianhj2021en_ZA
dc.description.sponsorshipThe National Research Foundationen_ZA
dc.description.urihttp://www.elsevier.com/locate/sajben_ZA
dc.identifier.citationAlberts, P.S.F. & Meyer, J.J.M. 2022, 'Integrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification : a case study on south-east African Erythroxylaceae species', South African Journal of Botany, vol. 146, pp. 174-186.en_ZA
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2021.10.005
dc.identifier.urihttp://hdl.handle.net/2263/82914
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2021 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 146, pp. 174-186, 2022. doi : 10.1016/j.sajb.2021.10.005.en_ZA
dc.subjectErythroxylumen_ZA
dc.subjectNectaropetalumen_ZA
dc.subjectGas chromatography-mass spectrometry (GC-MS)en_ZA
dc.subjectNuclear magnetic resonance (NMR)en_ZA
dc.subjectMetabolomicsen_ZA
dc.subjectDNA barcodingen_ZA
dc.titleIntegrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification : a case study on south-east African Erythroxylaceae speciesen_ZA
dc.typePostprint Articleen_ZA

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Alberts_Integrating_2022.pdf
Size:
1.37 MB
Format:
Adobe Portable Document Format
Description:
Postprint Article
Loading...
Thumbnail Image
Name:
Alberts_IntegratingSuppl_2022.pdf
Size:
669.81 KB
Format:
Adobe Portable Document Format
Description:
Supplementary Material

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: