Activity of South African medicinal plants against Listeria monocytogenes biofilms, and isolation of active compounds from Acacia karroo

dc.contributor.authorNyila, Monde Alfred
dc.contributor.authorLeonard, C.M.
dc.contributor.authorHussein, Ahmed A.
dc.contributor.authorLall, Namrita
dc.date.accessioned2017-01-12T05:58:41Z
dc.date.available2017-01-12T05:58:41Z
dc.date.issued2012-01
dc.description.abstractIn South Africa, the antimicrobial activity of many indigenous plants has been investigated. In general, studies have focused on planktonic bacteria, with less attention given to bacterial biofilms. Many organisms, however, including the opportunistic pathogen Listeria monocytogenes occur more frequently as biofilms. The aim of this study was to identify and select plants that exhibit the best antilisterial activity, isolate the bioactive compounds and determine their effect on the architecture of listerial biofilms. The ethyl acetate and chloroform extracts of thirteen plants were investigated for antilisterial activity. The ethyl acetate extract of Acacia karroo and Plectranthus ecklonii showed the best antilisterial activity, exhibiting a minimum inhibitory concentration (MIC) of 3.1 mg/ml and 0.5 mg/ml, respectively. These were further selected for the identification of bioactive compounds. Column chromatographic purification of the ethyl acetate extracts of the leaves of A. karroo led to the isolation of three known pure compounds, namely epicatechin (1), β-sitosterol (2) and epigallocatechin (3). Confocal scanning laser microscopy (CSLM) showed that the biomass of the listerial biofilm was reduced when the isolated compounds were added. The aggregation of cells that were exposed to β-sitosterol and epigallocatechin was reduced from 25 μm as observed in untreated cells to b10 μm in length.en_ZA
dc.description.departmentPlant Scienceen_ZA
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipThe University of Pretoria and National Research Foundation, South Africa.en_ZA
dc.description.urihttp://www.elsevier.com/locate/sajben_ZA
dc.identifier.citationNyila, MA, Leonard, CM, Hussein, AA & Lall, N 2012, 'Activity of South African medicinal plants against Listeria monocytogenes biofilms, and isolation of active compounds from Acacia karroo', South African Journal of Botany, vol. 78, pp. 220-227.en_ZA
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2011.09.001
dc.identifier.urihttp://hdl.handle.net/2263/58486
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2011 SAAB. Published by Elsevier B.V. Open Access funded by SAAB under a Creative Commons license.en_ZA
dc.subjectAntilisterial activityen_ZA
dc.subjectBiofilmsen_ZA
dc.subjectListeria monocytogenesen_ZA
dc.subjectMedicinal plantsen_ZA
dc.subjectConfocal scanning laser microscopy (CSLM)en_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectMinimum inhibitory concentration (MIC)en_ZA
dc.titleActivity of South African medicinal plants against Listeria monocytogenes biofilms, and isolation of active compounds from Acacia karrooen_ZA
dc.typeArticleen_ZA

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