Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions

dc.contributor.authorWu, YuQi
dc.contributor.authorZheng, HuoQing
dc.contributor.authorCorona, Miguel
dc.contributor.authorPirk, Christian Walter Werner
dc.contributor.authorMeng, Fei
dc.contributor.authorZheng, YuFei
dc.contributor.authorHu, FuLiang
dc.date.accessioned2017-09-21T09:59:08Z
dc.date.available2017-09-21T09:59:08Z
dc.date.issued2017-07-03
dc.description.abstractSecretions from mandibular glands (MGs) have important caste-specific functions that are associated with the social evolution of honey bees. To gain insights into the molecular architecture underlying these caste differences, we compared the gene expression patterns of MGs from queens, queenright workers (WQRs) and queenless workers (WQLs) using high-throughput RNA-sequencing technology. In total, we identified 46 candidate genes associated with caste-specific biosynthesis of fatty acid pheromones in the MG, including members of cytochrome P450 (CYP450) family and genes involved in fatty acid β-oxidation and ω-oxidation. For further identification of the CYP450s genes involved in the biosynthesis of MG secretions, we analyzed by means of qPCR, the expression levels of six of the CYP450 genes most abundantly expressed in the transcriptome analysis across different castes, ages, tasks and tissues. Our analysis revealed that CYP6AS8 and CYP6AS11, the most abundantly expressed CYP450 genes in worker and queen MGs, respectively, are selectively expressed in the MGs of workers and queens compared to other tissues. These results suggest that these genes might be responsible for the critical bifurcated hydroxylation process in the biosynthesis pathway. Our study contributes to the description of the molecular basis for the biosynthesis of fatty acid-derived pheromones in the MGs.en_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe National Natural Science Foundation of China (No. 31101773), the Science and Technology Department of Zhejiang Province, China (F.H., 2016C02054-11) and the earmarked funds for Modern Agroindustry Technology Research System (No. CARS-45).en_ZA
dc.description.urihttp://www.nature.com/scientificreportsen_ZA
dc.identifier.citationWu, Y.Q., Zheng, H.Q., Corona, M., Pirk, C., Meng, F., Zheng, Y.F. & Hu, F.L. 2017, 'Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions', Scientific Reports, vol. 7, no. 1, art. no. 4530, pp. 1-10.en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-017-04879-z
dc.identifier.urihttp://hdl.handle.net/2263/62494
dc.language.isoenen_ZA
dc.publisherNature Publishing Groupen_ZA
dc.rights© The Author(s) 2017. This is an open-access article distributed underthe terms of the Creative Commons Attribution License 4.0.en_ZA
dc.subjectSecretionsen_ZA
dc.subjectMandibular glandsen_ZA
dc.subjectHoneybee (Apis mellifera)en_ZA
dc.subjectAciden_ZA
dc.subjectProteinsen_ZA
dc.subjectCasteen_ZA
dc.subjectRNA-sequencingen_ZA
dc.subjectGene familyen_ZA
dc.subjectSocial insectsen_ZA
dc.subjectPheromone biosynthetic pathwayen_ZA
dc.subjectApolipophorin IIIen_ZA
dc.subjectBeta-oxidationen_ZA
dc.subjectDifferential expressionen_ZA
dc.titleComparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretionsen_ZA
dc.typeArticleen_ZA

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