Cytotoxicity and antimicrobial activity of isolated compounds from Monsonia angustifolia and Dodonaea angustifolia

dc.contributor.authorMcotshana, Zenande K.S.
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorKemboi, Douglas
dc.contributor.authorFouche, Gerda
dc.contributor.authorFamuyide, Ibukun Michael
dc.contributor.authorKrause, Rui W.M.
dc.contributor.authorSiwe-Noundou, Xavier
dc.contributor.authorTembu, Vuyelwa Jacqueline
dc.date.accessioned2023-05-24T07:36:29Z
dc.date.issued2023-01
dc.description.abstractETHNOPHARMACOLOGICAL RELEVANCE : Monsonia angustifolia is traditionally used to treat anthrax, heartburn, diarrhea, eye infections and hemorrhoids. Dodonaea angustifolia is frequently used as a treatment for dental pain, microbial infections and jungle fever. The two plant species were selected due to the presence of secondary metabolites such as coumarins, flavonoids, terpenoids, saponins and polyphenolics from the crude extracts, which exhibit pharmacological significance. The pure isolated compounds from the crude extracts are known for their diverse structures and interesting pharmacophores. AIM : To isolate and identify antibacterial and antifungal chemical constituents from Monsonia angustifolia and Dodonaea angustifolia plant extracts and evaluate the cytotoxicity of pure compounds from the crude extracts. MATERIALS AND METHODS : Extractives from M. angustifolia and D. angustifolia plants were isolated using chromatographic techniques and structures were elucidated based on NMR, IR and MS spectroscopic techniques. A microplate serial dilution method was used to evaluate the antibacterial activity of extracts and pure compounds against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and antifungal activity against Candida albicans and Cryptococcus neoformans. The cytotoxicity was determined using the 3-(4, 5-dimethylthiazol)-2, 5-diphenyl tetrazolium bromide (MTT) assay. RESULTS : The dichloromethane, ethyl acetate and methanol crude extracts from the plants exhibited significant inhibition of microbial growth. The phytochemical investigation of these active crude extracts led to the isolation of five pure active compounds, 5-methoxyjusticidin A (1), cis-phytyl diterpenoidal fatty acid ester (2), stigmasterol (3), β-sitosterol (4) and 5-hydroxy-7,4′-dimethoxyflavone (5). Stigmasterol (3) showed good antifungal activity against Cryptococcus neoformans with a minimum inhibition concentration (MIC) of 25 μg/mL and Candida albicans (MIC = 50 μg/mL). CONCLUSION : Compounds (1–5) isolated from Monsonia angustifolia and Dodonaea angustifolia showed antibacterial and antifungal activities and were non-toxic against Madin-Darby canine kidney (MDCK) cells and VERO monkey kidney (VERO) cells.en_US
dc.description.departmentChemistryen_US
dc.description.departmentParaclinical Sciencesen_US
dc.description.embargo2023-10-23
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe National Research Foundation (NRF) Thuthuka grant for financial assistance and the Tshwane University of Technology (Department of Chemistry), the University of Pretoria (Department of Paraclinical Sciences) and Rhodes University (Department of Chemistry) for supporting this research.en_US
dc.description.urihttps://www.elsevier.com/locate/jethpharmen_US
dc.identifier.citationMcotshana, Z.K.S, McGaw, L.J., Kemboi, D. et al. 2023, 'Cytotoxicity and antimicrobial activity of isolated compounds from Monsonia angustifolia and Dodonaea angustifolia', Journal of Ethnopharmacology, vol. 301, art. 115170, pp. 1-9, doi : 10.1016/j.jep.2022.115170.en_US
dc.identifier.issn0378-8741 (print)
dc.identifier.issn1872-7573 (online)
dc.identifier.other10.1016/j.jep.2022.115170
dc.identifier.urihttp://hdl.handle.net/2263/90790
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2022 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Ethnopharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Journal of Ethnopharmacology, vol. 301, art. 115170, pp. 1-9, 2023. doi : 10.1016/j.jep.2022.115170.en_US
dc.subjectMonsonia angustifoliaen_US
dc.subjectDodonaea angustifoliaen_US
dc.subjectAntibacterial activityen_US
dc.subjectAntifungal activityen_US
dc.subjectChemical constituentsen_US
dc.titleCytotoxicity and antimicrobial activity of isolated compounds from Monsonia angustifolia and Dodonaea angustifoliaen_US
dc.typePostprint Articleen_US

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