Ghibe river basin in Ethiopia : present situation of trypanocidal drug resistance in Trypanosoma congolense using test in mice and PCR-RFLP

dc.contributor.authorMoti, Y.
dc.contributor.authorFikru, R.
dc.contributor.authorVan den Abbeele, Jan
dc.contributor.authorBuscher, P.
dc.contributor.authorVan den Bossche, Peter
dc.contributor.authorDuchateau, L.
dc.contributor.authorDelespaux, Vincent
dc.date.accessioned2012-11-27T08:35:55Z
dc.date.available2012-11-27T08:35:55Z
dc.date.issued2012-10
dc.description.abstractA cross-sectional study was carried out in the Ghibe valley from August to October 2010. 411 head of cattle were sampled in eight villages for buffy coat examination (BCE) and blood spots were collected from each animal for trypanosomose diagnosis by 18S-PCR-RFLP and diminazene aceturate (DA) resistance by Ade2-PCR-RFLP. Three villages were selected in a zone where trypanosomosis control operations are currently on-going whereas the other 5 villages were located outside these control operations. Twenty-four samples (5.84%) were diagnosed positive for Trypanosoma congolense by BCE and injected in mice for further characterization. Twelve of those isolates successfully multiplied in mice and were tested by an in vivo mouse test for diminazene (DA) (10 and 20 mg/kg B.W.) and isometamidium (ISM) (1 mg/kg B.W.) resistance. All were shown to be resistant to both drugs at all doses. The use of the Ade2-PCR-RFLP on these isolates confirmed their DA-resistance profile. Seventythree of the collected blood spots (17.8%) were diagnosed positive for T. congolense by 18S-PCR-RFLP of which 37 (50.7%) gave amplification products with the Ade2-PCR-RFLP. Here, 35 (94.6%) showed a resistant profile, 1 (2.7%) a sensitive profile and 1 (2.7%) a mixed profile. The data were analysed by logistic regression model and the relapsing time in mice tests was assessed using the Cox regression model. There was no significant intervention effect (P = 0.83) with odds ratio equal to 1.21 when using the BCE data. 18S-PCR-RFLP test also showed no significant intervention effect (P = 0.60) with odds ratio equal to 1.43. The hazard ratio of getting parasitaemic after treatment with DA at 20 mg/kg B.W. compared to the control group was 0.38 which differs significantly from one (P < 0.001). Relapsing time after treatment with DA 10 mg/kg B.W. or ISM 1 mg/kg B.W. was also significantly longer than the prepatent period of the control group. The situation of drug resistance in the Ghibe valley is further discussed.en
dc.description.librarianab2012en
dc.description.sponsorshipThis research and the PhD scholarship of Moti Yohannes was financed by VLIR-UOS Institutional University Cooperation project with Jimma University. Fikru Regassa benefits from a PhD grant financed by the Belgian Directorate General for Development Cooperation.en
dc.description.urihttp://www.elsevier.com/locate/vetparen
dc.identifier.citationY Moti, R Fikru, J Van den Abbeele, P Buscher, P Van den Bossche, L. Duchateau & V Delespaux, Ghibe river basin in Ethiopia : present situation of trypanocidal drug resistance in Trypanosoma congolense using test in mice and PCR-RFLP, Veterinary Parasitology, vol. 189, no. 2-4, pp. 197-203 (2012), doi: 10.1016/j.vetpar.2012.04.022.en
dc.identifier.issn0304-4017 (print)
dc.identifier.issn1873-2550 (online)
dc.identifier.other10.1016/j.vetpar.2012.04.022
dc.identifier.urihttp://hdl.handle.net/2263/20544
dc.language.isoenen
dc.publisherElsevieren
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2012 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Parasitology, vol 189, issue 2-4, October 2012, doi: 10.1016/j.vetpar.2012.04.022.en
dc.subjectTrypanosoma congolenseen
dc.subjectIsometamidiumen
dc.subjectDiminazeneen
dc.subjectGhibe riveren
dc.subjectEthiopiaen
dc.subject.lcshTrypanosomaen
dc.subject.lcshPolymerase chain reactionen
dc.subject.lcshCattle -- Diseasesen
dc.subject.lcshDrug resistanceen
dc.titleGhibe river basin in Ethiopia : present situation of trypanocidal drug resistance in Trypanosoma congolense using test in mice and PCR-RFLPen
dc.typePostprint Articleen

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