Development of a genus-specific brucella real-time PCR assay targeting the 16S-23S rDNA internal transcribed spacer from Di erent specimen types
| dc.contributor.author | Nyarku, Rejoice | |
| dc.contributor.author | Hassim, Ayesha | |
| dc.contributor.author | Jonker, Annelize | |
| dc.contributor.author | Quan, Melvyn | |
| dc.contributor.email | melvyn.quan@up.ac.za | en_ZA |
| dc.date.accessioned | 2021-10-07T07:45:16Z | |
| dc.date.available | 2021-10-07T07:45:16Z | |
| dc.date.issued | 2020-11-11 | |
| dc.description.abstract | The aim of this study was to develop a 16S-23S ribosomal deoxyribonucleic acid internal transcribed spacer (ITS) quantitative polymerase chain reaction (qPCR) assay for the early diagnosis and rapid screening of brucellosis. Blood, milk, and tissue samples were spiked with B. abortus biovar 1 (B01988-18 strain) to determine the analytical sensitivity and specificity of the assay. The 95% limit of detection of the ITS qPCR assay was highest in tissue, followed by blood, then milk, i.e., 0.48, 4.43, and 15.18 bacteria/PCR reaction, respectively. The diagnostic performance of the assay was compared to the Brucella cell surface protein (BCSP) 31 qPCR assay and bacterial culture. Out of 56 aborted foetal tissue samples from bovine, ovine, and caprine, 33% (19/56) were positive for Brucella spp. The sensitivity and specificity of the ITS qPCR assay was 87% and 95% respectively, compared to 92% and 89% for the BCSP31 qPCR assay and 47% and 55% for bacterial culture, respectively. The assay was e cient, sensitive, and specific, making it a valuable tool in the early detection of the Brucella pathogen. | en_ZA |
| dc.description.department | Veterinary Tropical Diseases | en_ZA |
| dc.description.librarian | am2021 | en_ZA |
| dc.description.sponsorship | This work was supported by the Belgian Directorate General for Development Co-operation Framework Agreement (FA4 DGD/ITM 2017-2021) (Grant No. NRF: CSRP170522231749) awarded to the Department of Veterinary TropicalDiseases, aswell asRedMeatResearch andDevelopment, SouthAfrica (GrantNo.VET2018,0023). | en_ZA |
| dc.description.sponsorship | The Belgian Directorate General for Development Co-operation Framework Agreement (FA4 DGD/ITM 2017-2021) and Red Meat Research and Development, South Africa. | en_ZA |
| dc.description.uri | http://www.mdpi.com/journal/vetsci | en_ZA |
| dc.identifier.citation | Nyarku, R., Hassim, A., Jonker, A. et al. 2020, 'Development of a genus-specific brucella real-time PCR assay targeting the 16S-23S rDNA internal transcribed spacer from Di erent specimen types', Veterinary Sciences, vol. 7, art. 175, pp. 1-18. | en_ZA |
| dc.identifier.issn | 2306-7381 (online) | |
| dc.identifier.other | 10.3390/vetsci7040175 | |
| dc.identifier.uri | http://hdl.handle.net/2263/82067 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | MDPI | en_ZA |
| dc.rights | © 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_ZA |
| dc.subject | Diagnosis | en_ZA |
| dc.subject | Brucellosis | en_ZA |
| dc.subject | Blood | en_ZA |
| dc.subject | Milk | en_ZA |
| dc.subject | Abomasal fluid | en_ZA |
| dc.subject | Internal transcribed spacer (ITS) | en_ZA |
| dc.subject | Quantitative polymerase chain reaction (qPCR) | en_ZA |
| dc.subject | Brucella cell surface protein (BCSP) | en_ZA |
| dc.title | Development of a genus-specific brucella real-time PCR assay targeting the 16S-23S rDNA internal transcribed spacer from Di erent specimen types | en_ZA |
| dc.type | Article | en_ZA |