A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

dc.contributor.authorGana, James
dc.contributor.authorGcebe, Nomakorinte
dc.contributor.authorMoerane, Rebone
dc.contributor.authorNgoshe, Yusuf Bitrus
dc.contributor.authorTshuma, Tsakula
dc.contributor.authorMoabelo, Khomotso C.
dc.contributor.authorAdesiyun, Abiodun Adewale
dc.contributor.emailabiodun.adesiyun@up.ac.zaen_US
dc.date.accessioned2024-08-15T10:01:58Z
dc.date.available2024-08-15T10:01:58Z
dc.date.issued2024-02
dc.descriptionDATA AVAILABILITY : All the data are contained within the article.en_US
dc.description.abstractThese cross-sectional studies reported the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa. A total of 328 samples (faeces, feeds, silage, and drinking water) were collected from 23 cattle farms (communal, cow-calf, and feedlot), and 262 samples (faeces, carcass swabs, and effluents) from 8 beef abattoirs (low throughput and high throughput) were processed using standard bacteriological and molecular methods to detect Listeria species. The factors associated with the prevalence of Listeria species were investigated, and multiplex polymerase chain reaction (mPCR) was used to determine Listeria species, the pathogenic serogroups, and the carriage of eight virulence-associated genes by Listeria monocytogenes. The overall prevalence of Listeria species in cattle farms was 14.6%, comprising Listeria innocua (11.3%), Listeria monocytogenes (3.4%), Listeria welshimeri (0.0%) compared with 11.1%, comprising Listeria innocua (5.7%), Listeria monocytogenes (4.6%), Listeria welshimeri (0.8%) for beef abattoirs. Of the three variables (area, type of farm/abattoir, and sample type) investigated, only the sample types at abattoirs had a significant (P < 0.001) effect on the prevalence of L. innocua and L. welshimeri. The frequency of distribution of the serogroups based on 11 L. monocytogenes isolated from farms was 72.7% and 27.3% for the serogroup 1/2a-3a and 4b-4d-4e, respectively, while for the 12 L. monocytogenes isolates recovered from abattoirs, it was 25%, 8.3%, 50% and 16.7% for the serogroup 1/2a-3a, 1/2b-3b, 1/2c-3c, and 4b-4d-4e respectively (P < 0.05). All (100%) isolates of L. monocytogenes from the farms and abattoirs were positive for seven virulence genes (hlyA, inlB, plcA, iap, inlA, inlC, and inlJ). The clinical and food safety significance of the findings cannot be ignored.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-02:Zero Hungeren_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe Red Meat Research and Development, South Africa (RMRD-SA). Open access funding provided by University of Pretoria.en_US
dc.description.urihttp://link.springer.com/journal/11250en_US
dc.identifier.citationGana, J., Gcebe, N., Moerane, R. et al. A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa. Tropical Animal Health and Production 56, 88 (2024). https://doi.org/10.1007/s11250-024-03934-y.en_US
dc.identifier.issn0049-4747 (print)
dc.identifier.issn1573-7438 (online)
dc.identifier.other10.1007/s11250-024-03934-y
dc.identifier.urihttp://hdl.handle.net/2263/97658
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectListeriaen_US
dc.subjectSerogroupsen_US
dc.subjectVirulence genesen_US
dc.subjectCattle farmsen_US
dc.subjectAbattoirsen_US
dc.subjectSDG-02: Zero hungeren_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleA comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africaen_US
dc.typeArticleen_US

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