The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses
dc.contributor.author | Naalden, Diana | |
dc.contributor.author | Haegeman, Annelies | |
dc.contributor.author | De Almeida-Engler, Janice | |
dc.contributor.author | Birhane Eshetu, Firehiwot | |
dc.contributor.author | Bauters, Lander | |
dc.contributor.author | Gheysen Godelieve | |
dc.date.accessioned | 2018-10-17T11:08:05Z | |
dc.date.issued | 2018-11 | |
dc.description.abstract | On invasion of roots, plant‐parasitic nematodes secrete effectors to manipulate the cellular regulation of the host to promote parasitism. The root‐knot nematode Meloidogyne graminicola is one of the most damaging nematodes of rice. Here, we identified a novel effector of this nematode, named Mg16820, expressed in the nematode subventral glands. We localized the Mg16820 effector in the apoplast during the migration phase of the second‐stage juvenile in rice roots. In addition, during early development of the feeding site, Mg16820 was localized in giant cells, where it accumulated in the cytoplasm and the nucleus. Using transient expression in Nicotiana benthamiana leaves, we demonstrated that Mg16820 directed to the apoplast was able to suppress flg22‐induced reactive oxygen species production. In addition, expression of Mg16820 in the cytoplasm resulted in the suppression of the R2/Avr2‐ and Mi‐1.2‐induced hypersensitive response. A potential target protein of Mg16820 identified with the yeast two‐hybrid system was the dehydration stress‐inducible protein 1 (DIP1). Bimolecular fluorescence complementation resulted in a strong signal in the nucleus. DIP1 has been described as an abscisic acid (ABA)‐responsive gene and ABA is involved in the biotic and abiotic stress response. Our results demonstrate that Mg16820 is able to act in two cellular compartments as an immune suppressor and targets a protein involved in the stress response, therefore indicating an important role for this effector in parasitism. | en_ZA |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_ZA |
dc.description.department | Genetics | en_ZA |
dc.description.embargo | 2019-11-01 | |
dc.description.librarian | hj2018 | en_ZA |
dc.description.sponsorship | The Research Foundation Flanders FWO for financial support (FWO grants G010712N and 1502312N) and the Special Research Fund of Ghent University for the BOF13/GOA/030 project. The authors would also like to thank Lien De Smet for technical support, Dr John Jones for hosting Dr Annelies Haegeman at the James Hutton Institute (Dundee, UK), and Dr Miles Armstrong for teaching the yeast two-hybrid protocol during that stay, funded by COST Action 872 (COST-STSM-872-6657). The visit to INRA to perform the immunolocalization was funded by COST Action SUSTAIN FA1208. | en_ZA |
dc.description.uri | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1364-3703 | en_ZA |
dc.identifier.citation | Naalden, D., Haegeman, A., De Almeida-Engler, J. et al. 2018, 'The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses', Molecular Plant Pathology, vol. 19, no. 11, pp. 2416-2430. | en_ZA |
dc.identifier.issn | 1464-6722 (print) | |
dc.identifier.issn | 1364-3703 (online) | |
dc.identifier.other | 10.1111/mpp.12719 | |
dc.identifier.uri | http://hdl.handle.net/2263/66925 | |
dc.language.iso | en | en_ZA |
dc.publisher | Wiley | en_ZA |
dc.rights | © 2018 BSPP and John Wiley and Sons Ltd. This is the pre-peer reviewed version of the following article : 'The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses', Molecular Plant Pathology, vol. 19, no. 11, pp. 2416-2430, 2018, doi : 10.1111/mpp.12719. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1364-3703. | en_ZA |
dc.subject | Dehydration stress‐inducible protein 1 (DIP1) | en_ZA |
dc.subject | Root-knot nematodes | en_ZA |
dc.subject | Reactive oxygen species | en_ZA |
dc.subject | Parasitism | en_ZA |
dc.subject | Oryza sativa | en_ZA |
dc.subject | Nicotiana benthamiana | en_ZA |
dc.subject | Effector-triggered immunity | en_ZA |
dc.subject | PAMP-triggered immunity (PTI) | en_ZA |
dc.title | The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses | en_ZA |
dc.type | Postprint Article | en_ZA |