Purification of Cowdria ruminantium by density gradient centrifugation

dc.contributor.authorNeitz, Albert Walter Herman
dc.contributor.authorVermeulen, N.M.J.
dc.contributor.authorViljoen, G.J.
dc.contributor.editorBigalke, R.D.
dc.date.accessioned2017-11-13T11:31:40Z
dc.date.available2017-11-13T11:31:40Z
dc.date.created2017
dc.date.issued1987
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_ZA
dc.description.abstractThe isolation of Cowdria ruminantium by differential and isopycnic density gradient centrifugation is reviewed with special reference to the suitability of Percoll as density gradient medium. Infected sheep brain, Amblyomma hebraeum nymphae and various mouse organs were used as starting material. By these methods, partially purified viable populations of the organism with distinctly different densities were obtained. The conclusions are based upon results of analyses of density fractions by inoculation into sheep or mice, protein determination, electron microscopy and enzyme-linked immunosorbent assay. Morphological differences were observed in the density fractions obtained from infected brain tissue and A. hebraeum.en_ZA
dc.identifier.citationNeitz, AWH, Vermeulen, NMJ & Viljoen, GJ 1987, 'Purification of Cowdria ruminantium by density gradient centrifugation', Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 223-231.en_ZA
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/63125
dc.language.isoenen_ZA
dc.publisherPublished by the Government Printer, Pretoria.en_ZA
dc.rights©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).en_ZA
dc.subjectVeterinary medicineen_ZA
dc.subject.lcshVeterinary medicine -- South Africa
dc.titlePurification of Cowdria ruminantium by density gradient centrifugationen_ZA
dc.title.alternativeHeartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986en_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
43neitz1987.pdf
Size:
450.17 KB
Format:
Adobe Portable Document Format
Description:
Main article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: