Cellular and molecular targets of waterbuck repellent blend odors in antennae of Glossina fuscipes fuscipes Newstead, 1910

dc.contributor.authorDiallo, Souleymane
dc.contributor.authorShahbaaz, Mohd
dc.contributor.authorTorto, Baldwyn
dc.contributor.authorChristoffels, Alan
dc.contributor.authorMasiga, Daniel
dc.contributor.authorGetahun, Merid N.
dc.date.accessioned2020-10-06T06:18:18Z
dc.date.available2020-10-06T06:18:18Z
dc.date.issued2020-06-03
dc.descriptionTable S1 : Primers used for RT-qPCR experiments.en_ZA
dc.descriptionTable S2 : Protein quality check and 3-D structure validation using ProSA Z-score and Ramachandran plot statistics obtained from Procheck.en_ZA
dc.descriptionTable S3 : Alteration of G. f. fuscipes OR mRNA and D. melanogaster vs G. f. fuscipes orthologs genes response comparison.en_ZA
dc.description.abstractInsects that transmit many of the world’s deadliest animal diseases, for instance trypanosomosis, find their suitable hosts and avoid non-preferred hosts mostly through olfactory cues. The waterbuck repellent blend (WRB) comprising geranylacetone, guaiacol, pentanoic acid, and d-octalactone derived from waterbuck skin odor is a repellent to some savannah-adapted tsetse flies and reduces trap catches of riverine species. However, the cellular and molecular mechanisms associated with detection and coding of the repellent odors remain to be elucidated. Here, we demonstrated that WRB inhibited blood feeding in both Glossina pallidipes Austen, 1903 and Glossina fuscipes fuscipes Newstead, 1910. Using the DREAM (Deorphanization of Receptors based on Expression Alterations in odorant receptor mRNA levels) technique, combined with ortholog comparison and molecular docking, we predicted the putative odorant receptors (ORs) for the WRB in G. f. fuscipes, a non-model insect. We show that exposure of G. f. fuscipes in vivo to WRB odorant resulted in up- and downregulation of mRNA transcript of several ORs. The WRB component with strong feeding inhibition altered mRNA transcript differently as compared to an attractant odor, showing these two odors of opposing valence already segregate at the cellular and molecular levels. Furthermore, molecular dynamics simulations demonstrated that the predicted ligand– OR binding pockets consisted mostly of hydrophobic residues with a few hydrogen bonds but a stable interaction. Finally, our electrophysiological response showed the olfactory sensory neurons of G. f. fuscipes tuned to the tsetse repellent components in different sensitivity and selectivity.en_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.librarianam2020en_ZA
dc.description.urihttp://www.frontiersin.org/Cellular_Neuroscienceen_ZA
dc.identifier.citationDiallo S., Shahbaaz M., Torto B., Christoffels A., Masiga D. and Getahun M.N. (2020) Cellular and Molecular Targets of Waterbuck Repellent Blend Odors in Antennae of Glossina fuscipes fuscipes Newstead, 1910. Frontiers in Cellular Neuroscience 14:137. DOI: 10.3389/fncel.2020.00137.en_ZA
dc.identifier.issn1662-5102 (online)
dc.identifier.other10.3389/fncel.2020.00137
dc.identifier.urihttp://hdl.handle.net/2263/76351
dc.language.isoenen_ZA
dc.publisherFrontiers Mediaen_ZA
dc.rights© 2020 Diallo, Shahbaaz, Torto, Christoffels, Masiga and Getahun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en_ZA
dc.subjectBehavioren_ZA
dc.subjectMolecular dockingen_ZA
dc.subjectMolecular dynamicsen_ZA
dc.subjectOlfactionen_ZA
dc.subjectPhysiologyen_ZA
dc.subjectRepellentsen_ZA
dc.subjectTsetseen_ZA
dc.subjectWaterbuck repellent blend (WRB)en_ZA
dc.titleCellular and molecular targets of waterbuck repellent blend odors in antennae of Glossina fuscipes fuscipes Newstead, 1910en_ZA
dc.typeArticleen_ZA

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