Population structure of non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africa

dc.contributor.authorMatle, Itumeleng
dc.contributor.authorMafuna, Thendo
dc.contributor.authorMadoroba, Evelyn
dc.contributor.authorMbatha, Khanyisile R.
dc.contributor.authorMagwedere, Kudakwashe
dc.contributor.authorPierneef, Rian Ewald
dc.date.accessioned2020-12-22T08:50:51Z
dc.date.available2020-12-22T08:50:51Z
dc.date.issued2020-08
dc.descriptionSupplementary Materials: Table S1: Listeria pathogenic islands metadata, Table S2: InlA protein sequence clusters metadata.en_ZA
dc.description.abstractMeat products have been implicated in many listeriosis outbreaks globally, however there is a dearth of information on the diversity of L. monocytogenes isolates circulating in food products in South Africa. The aim of this study was to investigate the population structure of L. monocytogenes isolated in the meat value chain within the South African market. Based on whole-genome sequence analysis, a total of 217 isolates were classified into two main lineage groupings namely lineages I (n = 97; 44.7%) and II (n = 120; 55.3%). The lineage groups were further differentiated into IIa (n = 95, 43.8%), IVb (n = 69, 31.8%), IIb (n = 28, 12.9%), and IIc (n = 25, 11.5%) sero-groups. The most abundant sequence types (STs) were ST204 (n = 32, 14.7%), ST2 (n = 30, 13.8%), ST1 (n = 25, 11.5%), ST9 (n = 24, 11.1%), and ST321 (n = 21, 9.7%). In addition, 14 clonal complex (CCs) were identified with over-representation of CC1, CC3, and CC121 in “Processed Meat-Beef”, “RTE-Poultry”, and “Raw-Lamb” meat categories, respectively. Listeria pathogenic islands were present in 7.4% (LIPI-1), 21.7% (LIPI-3), and 1.8% (LIPI-4) of the isolates. Mutation leading to premature stop codons was detected in inlA virulence genes across isolates identified as ST121 and ST321. The findings of this study demonstrated a high-level of genomic diversity among L. monocytogenes isolates recovered across the meat value chain control points in South Africa.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianpm2020en_ZA
dc.description.sponsorshipDepartment of Agriculture, Land Reform and Rural Development (DALRRD)en_ZA
dc.description.urihttp://www.mdpi.com/journal/microorganismsen_ZA
dc.identifier.citationMatle, I., Mafuna, T., Madoroba, E. et al. 2020, 'Population structure of Non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africa', Microorganisms, vol. 8, no. 8, art. 1152, pp. 1018.en_ZA
dc.identifier.issn2076-2607 (online)
dc.identifier.other10.3390/microorganisms8081152
dc.identifier.urihttp://hdl.handle.net/2263/77465
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_ZA
dc.subjectL. monocytogenesen_ZA
dc.subjectSubtypingen_ZA
dc.subjectSerogroupsen_ZA
dc.subjectSequence typesen_ZA
dc.subjectClone complexesen_ZA
dc.subjectPathogenic islandsen_ZA
dc.subjectLineagesen_ZA
dc.subjectInlAen_ZA
dc.subjectSequencingen_ZA
dc.subjectRed meaten_ZA
dc.subjectPoultryen_ZA
dc.subjectValue chainen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectPopulation structureen_ZA
dc.subjectListeria monocytogenesen_ZA
dc.titlePopulation structure of non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africaen_ZA
dc.typeArticleen_ZA

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