Population structure of non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africa
dc.contributor.author | Matle, Itumeleng | |
dc.contributor.author | Mafuna, Thendo | |
dc.contributor.author | Madoroba, Evelyn | |
dc.contributor.author | Mbatha, Khanyisile R. | |
dc.contributor.author | Magwedere, Kudakwashe | |
dc.contributor.author | Pierneef, Rian Ewald | |
dc.date.accessioned | 2020-12-22T08:50:51Z | |
dc.date.available | 2020-12-22T08:50:51Z | |
dc.date.issued | 2020-08 | |
dc.description | Supplementary Materials: Table S1: Listeria pathogenic islands metadata, Table S2: InlA protein sequence clusters metadata. | en_ZA |
dc.description.abstract | Meat products have been implicated in many listeriosis outbreaks globally, however there is a dearth of information on the diversity of L. monocytogenes isolates circulating in food products in South Africa. The aim of this study was to investigate the population structure of L. monocytogenes isolated in the meat value chain within the South African market. Based on whole-genome sequence analysis, a total of 217 isolates were classified into two main lineage groupings namely lineages I (n = 97; 44.7%) and II (n = 120; 55.3%). The lineage groups were further differentiated into IIa (n = 95, 43.8%), IVb (n = 69, 31.8%), IIb (n = 28, 12.9%), and IIc (n = 25, 11.5%) sero-groups. The most abundant sequence types (STs) were ST204 (n = 32, 14.7%), ST2 (n = 30, 13.8%), ST1 (n = 25, 11.5%), ST9 (n = 24, 11.1%), and ST321 (n = 21, 9.7%). In addition, 14 clonal complex (CCs) were identified with over-representation of CC1, CC3, and CC121 in “Processed Meat-Beef”, “RTE-Poultry”, and “Raw-Lamb” meat categories, respectively. Listeria pathogenic islands were present in 7.4% (LIPI-1), 21.7% (LIPI-3), and 1.8% (LIPI-4) of the isolates. Mutation leading to premature stop codons was detected in inlA virulence genes across isolates identified as ST121 and ST321. The findings of this study demonstrated a high-level of genomic diversity among L. monocytogenes isolates recovered across the meat value chain control points in South Africa. | en_ZA |
dc.description.department | Biochemistry | en_ZA |
dc.description.department | Genetics | en_ZA |
dc.description.department | Microbiology and Plant Pathology | en_ZA |
dc.description.librarian | pm2020 | en_ZA |
dc.description.sponsorship | Department of Agriculture, Land Reform and Rural Development (DALRRD) | en_ZA |
dc.description.uri | http://www.mdpi.com/journal/microorganisms | en_ZA |
dc.identifier.citation | Matle, I., Mafuna, T., Madoroba, E. et al. 2020, 'Population structure of Non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africa', Microorganisms, vol. 8, no. 8, art. 1152, pp. 1018. | en_ZA |
dc.identifier.issn | 2076-2607 (online) | |
dc.identifier.other | 10.3390/microorganisms8081152 | |
dc.identifier.uri | http://hdl.handle.net/2263/77465 | |
dc.language.iso | en | en_ZA |
dc.publisher | MDPI | en_ZA |
dc.rights | © 2020 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en_ZA |
dc.subject | L. monocytogenes | en_ZA |
dc.subject | Subtyping | en_ZA |
dc.subject | Serogroups | en_ZA |
dc.subject | Sequence types | en_ZA |
dc.subject | Clone complexes | en_ZA |
dc.subject | Pathogenic islands | en_ZA |
dc.subject | Lineages | en_ZA |
dc.subject | InlA | en_ZA |
dc.subject | Sequencing | en_ZA |
dc.subject | Red meat | en_ZA |
dc.subject | Poultry | en_ZA |
dc.subject | Value chain | en_ZA |
dc.subject | South Africa (SA) | en_ZA |
dc.subject | Population structure | en_ZA |
dc.subject | Listeria monocytogenes | en_ZA |
dc.title | Population structure of non-ST6 Listeria monocytogenes isolated in the red meat and poultry value chain in South Africa | en_ZA |
dc.type | Article | en_ZA |
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