Mosquitoes larvicidal activity of Ocimum kilimandscharicum oil formulation under laboratory and field-simulated conditions

dc.contributor.authorOchola, John Bwire
dc.contributor.authorMutero, Clifford Maina
dc.contributor.authorMarubu, Rose Muthoni
dc.contributor.authorHaller, Barbara Frei
dc.contributor.authorHassanali, Ahmed
dc.contributor.authorLwande, Wilber
dc.date.accessioned2023-08-11T10:41:09Z
dc.date.available2023-08-11T10:41:09Z
dc.date.issued2022-02-16
dc.descriptionDATA AVAILABILITY STATEMENT : All data generated or analyzed during this study are included in this manuscript.en_US
dc.description.abstractMosquitoes are vectors of many severe diseases, including malaria, yellow as well as dengue fever, and lymphatic filariasis. The use of synthetic chemical insecticides for mosquito control has been associated with resistance development and detrimental human, and ecological effects. For a safer alternative, the emulsified Ocimum kilimandscharicum oil formulation was evaluated for its larvicidal activity. The oil was analyzed by GC and GC/MS. The formulations were evaluated against third instar mosquito larvae in the laboratory and later compared with Bacillus thuringiensis subsp. israelensis against An. gambiae under field-simulated conditions. Thirty-nine compounds were identified in the oil, the main ones being D-camphor (36.6%) and limonene (18.6%). The formulation showed significant larval mortalities against An. gambiae and An. arabiensis larvae with LC50 of 0.07 and 0.31 ppm, respectively, at 24 h. Under the field-simulated trial, within 24 h, the formulation showed 98% mortality while Bti had achieved 54%. On day three, it caused 100% mortality while Bti achieved 76.5%. The high bioactivity and sublethal toxic effects to offspring of treated mosquito larvae, in terms of disruption of larval morphological aspects, suggest the high potential of the formulation as a botanical larvicide. The formulation, thus, may provide a valuable alternative for the effective and eco-friendly control of disease vectors.en_US
dc.description.departmentSchool of Health Systems and Public Health (SHSPH)en_US
dc.description.departmentUP Centre for Sustainable Malaria Control (UP CSMC)en_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe Biovision Foundation Switzerland. The article processing charge (APC) was funded by: Global Environment Facility.en_US
dc.description.urihttps://www.mdpi.com/journal/insectsen_US
dc.identifier.citationOchola, J.B.; Mutero, C.M.; Marubu, R.M.; Haller, B.F.; Hassanali, A.; Lwande,W. Mosquitoes Larvicidal Activity of Ocimum kilimandscharicum Oil Formulation under Laboratory and Field-Simulated Conditions. Insects 2022, 13, 203. https://DOI.org/10.3390/insects13020203.en_US
dc.identifier.issn2075-4450 (online)
dc.identifier.other10.3390/insects13020203
dc.identifier.urihttp://hdl.handle.net/2263/91887
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectLarvicideen_US
dc.subjectMalariaen_US
dc.subjectMosquito controlen_US
dc.subjectBotanicalen_US
dc.subjectFormulationen_US
dc.subjectBiopesticideen_US
dc.titleMosquitoes larvicidal activity of Ocimum kilimandscharicum oil formulation under laboratory and field-simulated conditionsen_US
dc.typeArticleen_US

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