Development of a nested quantitative real-time PCR for detecting Phytophthora cinnamomi in Persea americana rootstocks

dc.contributor.authorEngelbrecht, J.
dc.contributor.authorDuong, Tuan A.
dc.contributor.authorVan den Berg, Noelani
dc.contributor.emailnoelani.vandenberg@up.ac.zaen_US
dc.date.accessioned2013-10-02T07:02:26Z
dc.date.available2013-10-02T07:02:26Z
dc.date.issued2013
dc.description.abstractPhytophthora cinnamomi causes Phytophthora root rot (PRR) in avocado (Persea americana), an important disease that causes severe economic losses to the avocado industry globally. To date,no PRR-resistant avocado rootstock variety has been discovered, although certain rootstock varieties have been shown to be more tolerant than others. In this study we developed an accurate, low cost assay for in planta quantification of P. cinnamomi to evaluate disease tolerance. A nested real time PCR assay was developed to sensitively detect pathogen DNA in plant tissues. Root samples from a highly tolerant (Dusa®) and less tolerant (R0.12) rootstock were collected at 0, 3, 7, 14 and 21 days after inoculation with P. cinnamomi and used for pathogen quantification. Nested primers developed in this study were specific and sensitive and could detect P. cinnamomi in root tissues. The amount of P. cinnamomi quantified in roots was significantly higher in the less tolerant R0.12 plants when compared to the highly tolerant Dusa® plants at all time points. This study has confirmed the known status of disease tolerance of Dusa® and R0.12 avocado rootstocks in a quantitative manner and provides a reliable molecular tool to assist with industry breeding programs for the selection of PRR-resistant avocado rootstock varieties.en_US
dc.description.librarianhb2013en_US
dc.description.sponsorshipThe Hans Merensky Foundation and the THRIP programme of the National Research Foundation of South Africa.en_US
dc.description.urihttp://www.apsnet.org/en_US
dc.identifier.citationEngelbrecht, J, Duong, TA & Van den Berg, N 2013 , 'Development of a nested quantitative real-time PCR for detecting Phytophthora cinnamomi in Persea americana rootstocks', Plant Disease, vol. 97, no. 8, pp. 1012-1017.en_US
dc.identifier.issn0191-2917 (print)
dc.identifier.issn1943-7692 (online)
dc.identifier.other10.1094/PDIS-11-12-1007-RE
dc.identifier.urihttp://hdl.handle.net/2263/31868
dc.language.isoenen_US
dc.publisherAmerican Phytopathological Societyen_US
dc.rights© 2013 The American Phytopathological Societyen_US
dc.subjectPhytophthora cinnamomien_US
dc.subjectPhytophthora root rot (PRR)en_US
dc.subjectAvocado (Persea americana)en_US
dc.titleDevelopment of a nested quantitative real-time PCR for detecting Phytophthora cinnamomi in Persea americana rootstocksen_US
dc.typePostprint Articleen_US

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