Iron and carbon monoxide enhance coagulation and attenuate fibrinolysis by different mechanisms

dc.contributor.authorNielsen, Vance G.
dc.contributor.authorPretorius, Etheresia
dc.date.accessioned2014-12-08T06:22:06Z
dc.date.issued2014-10
dc.description.abstractTwo parallel lines of investigation elucidating novel mechanisms by which iron (scanning electron microscopy-based) and carbon monoxide (viscoelastic-based) enhance coagulation and diminish fibrinolysis have emerged over the past few years. However, a multimodal approach to ascertain the effects of iron and carbon monoxide remained to be performed. Such investigation could be important, as iron and carbon monoxide are two of the products of heme catabolism via heme oxygenase-1, an enzyme upregulated in a variety of disease states associated with thrombophilia. Human plasma was exposed to ferric chloride, carbon monoxide derived from carbon monoxide-releasing molecule-2, or their combination. Viscoelastic studies demonstrated ferric chloride and carbon monoxide mediated enhancement of velocity of growth, and final clot strength, with the combination of the two molecules noted to have all the prothrombotic kinetic effects of either separately. Parallel ultrastructural studies demonstrated separate types of fibrin polymer cross-linking and matting in plasma exposed to ferric chloride and carbon monoxide, with the combination sharing features of each molecule. In conclusion, we present the first evidence that iron and carbon monoxide interact with key coagulation and fibrinolytic processes, resulting in thrombi that begin to form more quickly, grow faster, become stronger, and are more resistant to lysis.en_US
dc.description.embargo2015-10-30
dc.description.librarianhj2014en_US
dc.description.urihttp://journals.lww.com/bloodcoagulationen_US
dc.identifier.citationNielsen, VG & Pretorius, E 2014, 'Iron and carbon monoxide enhance coagulation and attenuate fibrinolysis by different mechanisms', Blood Coagulation and Fibrinolysis, vol. 25, no. 7, pp. 695-702.en_US
dc.identifier.issn0957-5235 (print)
dc.identifier.issn1473-5733 (online)
dc.identifier.other10.1097/MBC.0000000000000128
dc.identifier.urihttp://hdl.handle.net/2263/42849
dc.language.isoenen_US
dc.publisherLippincott Williams and Wilkinsen_US
dc.rights© 2014 Wolters Kluwer Health / Lippincott Williams & Wilkins. This is a non-final version of an article published in final form in Blood Coagulation and Fibrinolysis, vol. 25, no.7, pp. 695-702, 2014. doi : 10.1097/MBC.0000000000000128.en_US
dc.subjectCarbon monoxide (CO)en_US
dc.subjectCoagulationen_US
dc.subjectFibrinogenen_US
dc.subjectFibrinolysisen_US
dc.subjectIronen_US
dc.titleIron and carbon monoxide enhance coagulation and attenuate fibrinolysis by different mechanismsen_US
dc.typePostprint Articleen_US

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