Assessment of gene flow between gossypium hirsutum and G. herbaceum : evidence of unreduced gametes in the diploid progenitor

dc.contributor.authorMontes, E.
dc.contributor.authorCoriton, O.
dc.contributor.authorEber, F.
dc.contributor.authorHuteau, V.
dc.contributor.authorLacape, J.M.
dc.contributor.authorReinhardt, Carl Frederick (Charlie)
dc.contributor.authorMarais, Diana
dc.contributor.authorHofs, J.L.
dc.contributor.authorChevre, A.M.
dc.contributor.authorPannetier, C.
dc.date.accessioned2017-09-01T06:55:53Z
dc.date.available2017-09-01T06:55:53Z
dc.date.issued2017-07-25
dc.description.abstractIn the framework of a gene flow assessment, we investigated the natural hybridization rate between Gossypium hirsutum (AADD genome) and G. herbaceum (AA genome). The latter species, a diploid progenitor of G. hirsutum, is spontaneously present in South Africa. Reciprocal crosses were performed without emasculation between G. herbaceum and G. hirsutum. Neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the G. hirsutum · G. herbaceum cross showed any hybrid features. Of the 148 plants produced from the G. herbaceum · G. hirsutum cross, three showed a hybrid phenotype, and their hybrid status was confirmed by SSR markers. Analysis of DNA content by flow cytometry and morphological traits clearly showed that two of these plants were triploid (AAD). The third plant had a flow cytometry DNA content slightly higher than G. hirsutum. In addition, its morphological characteristics (plant architecture, presence and size of petal spots, leaf shape) led us to conclude that this plant was AAAD thus resulting from fertilization with an unreduced AA gamete of the female G. herbaceum parent. Fluorescent In Situ Hybridization (FISH) and meiotic behavior confirmed this hypothesis. To the best of our knowledge, this is the first description of such gametes in G. herbaceum, and it opens new avenues in breeding programs. Furthermore, this plant material could provide a useful tool for studying the expression of genes duplicated in the A and D cotton genome.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThey acknowledge support for this project from the French Ministry for Education and Research/ ANR (Agence Nationale de la Recherche), grant MFD/AO OGM 0217. The Jean-Pierre Bourgin Institute (IJPB) benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).en_ZA
dc.description.urihttp://www.g3journal.orgen_ZA
dc.identifier.citationMontes, E., Coriton, O., Eber, F., Huteau, V., Lacape, J.M., Reinhardt, C., Marais, D., Hofs, J.L., Chevre, A.M. & Pannetier, C. 2017, 'Assessment of gene flow between gossypium hirsutum and G. herbaceum : evidence of unreduced gametes in the diploid progenitor', G3: Genes, Genomes, Genetics, vol. 7, pp. 2185-2193.en_ZA
dc.identifier.issn2160-1836 (online)
dc.identifier.other10.1534/g3.117.041509
dc.identifier.urihttp://hdl.handle.net/2263/62164
dc.language.isoenen_ZA
dc.publisherGenetics Society of Americaen_ZA
dc.rights© 2017 Montes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectGene flowen_ZA
dc.subjectNatural hybridizationen_ZA
dc.subjectUnreduced gameteen_ZA
dc.subjectGossypium hirsutumen_ZA
dc.subjectGossypium herbaceumen_ZA
dc.titleAssessment of gene flow between gossypium hirsutum and G. herbaceum : evidence of unreduced gametes in the diploid progenitoren_ZA
dc.typeArticleen_ZA

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