Comparison of procedures to evaluate the pathogenicity of Ceratocystis fimbriata sensu lato isolates from Eucalyptus in South Africa

dc.contributor.authorVan Wyk, Marelize
dc.contributor.authorHeath, R.N. (Ronald Natale)
dc.contributor.authorTarigan, Marthin
dc.contributor.authorVermeulen, Marcele
dc.contributor.authorWingfield, Michael J.
dc.date.accessioned2011-02-22T06:23:46Z
dc.date.available2011-02-22T06:23:46Z
dc.date.issued2010-06
dc.description.abstractCeratocystis fimbriata sensu lato (s.l.) is an important pathogen of Eucalyptus. Pathogenicity of isolates has typically been evaluated by inoculating seedlings under greenhouse conditions. It is, however, not clear how accurately this reflects pathogenicity under field conditions. In this study, five techniques to potentially screen C. fimbriata isolates for their relative pathogenicity to Eucalyptus were compared. These included: in vitro growth comparisons on artificial media; inoculations on apples; inoculation on Eucalyptus seedlings in a greenhouse; inoculations on Eucalyptus bolts freshly cut from stems of young trees; and field inoculations on young trees. Eight isolates of C. fimbriata s.l. collected from various areas in South Africa were used. There was considerable variation in growth in culture and aggressiveness of the eight isolates. Field inoculations on young trees were best correlated with inoculations of bolts (r = 0.76). Lower correlation coefficients were obtained with seedlings (r = 0.59), apple inoculations (r = 0.56), and in vitro colony growth (r = 0.42). Inoculation of bolts provides a rapid and reliable method to screen isolates of C. fimbriata s.l. for pathogenicity to Eucalyptus.en
dc.description.sponsorshipNational Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP), THRIP initiative of the Department of Trade and Industry.en_US
dc.identifier.citationVan Wyk, M, Heath, RN, Tarigan, M, Vermeulen, M & Wingfield, MJ 2010, 'Comparison of procedures to evaluate the pathogenicity of Ceratocystis fimbriata sensu lato isolates from Eucalyptus in South Africa', Southern Forests, vol. 72, no. 2, pp. 57-62. [http://www.nisc.co.za/journals?id=11]en
dc.identifier.issn2070-2620
dc.identifier.issn2070-2639 (online)
dc.identifier.other10.2989/20702620.2010.507011
dc.identifier.urihttp://hdl.handle.net/2263/15892
dc.language.isoenen_US
dc.publisherNational Inquiry Services Centre (NISC)en_US
dc.rights© NISC (Pty) Ltd. This is the accepted, peer‐reviewed manuscript of an article whose final and definitive form has been published in Southern Forests, 2010, vol. 72, no. 2, pp. 57-62.en_US
dc.subjectPathogenicityen
dc.subjectIsolatesen
dc.subject.lcshCeratocystisen
dc.subject.lcshPathogenic microorganisms -- South Africa -- Identificationen
dc.subject.lcshEucalyptus -- South Africaen
dc.titleComparison of procedures to evaluate the pathogenicity of Ceratocystis fimbriata sensu lato isolates from Eucalyptus in South Africaen
dc.typePostprint Articleen

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
VanWyk_Comparison(2010).pdf
Size:
223.65 KB
Format:
Adobe Portable Document Format
Description:
Postprint Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
2.43 KB
Format:
Item-specific license agreed upon to submission
Description: