Laboratory evaluation of the BioFire FilmArray Pneumonia plus panel compared to conventional methods for the identification of bacteria in lower respiratory tract specimens : a prospective cross-sectional study from South Africa

dc.contributor.authorMitton, Barend C.
dc.contributor.authorRule, Roxanne
dc.contributor.authorSaid, H.M. (Halima Mohammed)
dc.date.accessioned2021-06-23T07:45:52Z
dc.date.issued2021-02
dc.description.abstractLower respiratory tract infections are important causes of morbidity and mortality. The global increase in antimicrobial resistance necessitates rapid diagnostic assays. The BioFire FilmArray Pneumonia plus (FAPP) panel is a Food and Drug Administration-approved multiplex polymerase chain reaction assay that detects the most important etiological agents of pneumonia and associated antibiotic resistance genes, in approximately 1 hour. This study assessed the diagnostic performance of this assay by comparing it to conventional culture methods in the analysis of 59 lower respiratory tract specimens. The sensitivity and specificity of the FAPP panel for bacterial detection were 92.0% (95% confidence interval [CI], 80.8% to 97.8%) and 93.8% (95% CI, 91.1% to 95.3%) respectively. For detecting antibiotic resistance, the positive- and negative percent agreement were 100% (95% CI, 81.5% to 100.0%) and 98.5% (95% CI, 216 96.7% to 99.4%) respectively. The FAPP panel was found to be highly accurate in evaluating tracheal aspirate specimens from hospitalized patients.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.embargo2021-10-10
dc.description.librarianhj2021en_ZA
dc.description.sponsorshipBioMérieuxen_ZA
dc.description.urihttp://www.elsevier.com/locate/diagmicrobioen_ZA
dc.identifier.citationMitton, B., Rule, R. & Said, M. 2021, 'Laboratory evaluation of the BioFire FilmArray Pneumonia plus panel compared to conventional methods for the identification of bacteria in lower respiratory tract specimens : a prospective cross-sectional study from South Africa', Diagnostic Microbiology and Infectious Disease, vol. 99, no. 2, art. 115236, pp. 1-6.en_ZA
dc.identifier.issn0732-8893 (print)
dc.identifier.issn1879-0070 (online)
dc.identifier.other10.1016/j.diagmicrobio.2020.115236
dc.identifier.urihttp://hdl.handle.net/2263/80548
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2020 Elsevier Inc.. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Diagnostic Microbiology and Infectious Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Diagnostic Microbiology and Infectious Diseases, vol. 99, no. 2, art. 115236, pp. 1-6, 2021. doi : 10.1016/j.diagmicrobio.2020.115236.en_ZA
dc.subjectBioFireen_ZA
dc.subjectFilmArray Pneumonia plus (FAPP)en_ZA
dc.subjectPneumoniaen_ZA
dc.subjectAntimicrobial resistance (AMR)en_ZA
dc.subjectInfection prevention and control (IPC)en_ZA
dc.subjectMultiplex PCRen_ZA
dc.subjectLower respiratory tract infection (LRTI)en_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.titleLaboratory evaluation of the BioFire FilmArray Pneumonia plus panel compared to conventional methods for the identification of bacteria in lower respiratory tract specimens : a prospective cross-sectional study from South Africaen_ZA
dc.typePostprint Articleen_ZA

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