In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells
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Authors
Josemans, A.I.
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Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.
Abstract
The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for reliable growth of E. ruminantium. Growth of the Welgevonden stock in BHK and CHO-K1 cells could lead to the development of suspension cultures suitable for the mass production of E. ruminantium for an inactivated elementary body vaccine.
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Keywords
Veterinary medicine, BHK cells, CHO-K1 cells, Cycloheximide, Ehrlichia (Cowdria) ruminantium, Heartwater, In vitro cultivation, MDBK cells
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Citation
Zweygarth, E & Josemans, AI 2003, 'In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells'. Onderstepoort Journal of Veterinary Research, vol. 70, no. 2, pp. 165-168.