A survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenya

dc.contributor.authorChiuya, Tatenda
dc.contributor.authorMasiga, Daniel K.
dc.contributor.authorFalzonI, Laura C.
dc.contributor.authorBastos, Armanda D.S.
dc.contributor.authorFèvre, Eric M.
dc.contributor.authorVillinger, Jandouwe
dc.date.accessioned2022-03-18T07:02:24Z
dc.date.available2022-03-18T07:02:24Z
dc.date.issued2021-05
dc.description.abstractAedes aegypti and Culex pipiens complex mosquitoes are prolific vectors of arboviruses that are a global threat to human and animal health. Increased globalization and ease of travel have facilitated the worldwide dissemination of these mosquitoes and the viruses they transmit. To assess disease risk, we determined the frequency of arboviruses in western Kenyan counties bordering an area of high arboviral activity. In addition to pathogenic viruses, insect-specific flaviviruses (ISFs), some of which are thought to impair the transmission of specific pathogenic arboviruses, were also evaluated. We trapped mosquitoes in the short and long rainy seasons in 2018 and 2019 at livestock markets and hospitals. Mosquitoes were screened for dengue, chikungunya and other human pathogenic arboviruses, ISFs, and their blood-meal sources as determined by high-resolution melting analysis of (RT-)PCR products. Of 6,848 mosquitoes collected, 89% were trapped during the long rainy season, with A. aegypti (59%) and Cx. pipiens sensu lato (40%) being the most abundant. Most blood-fed mosquitoes were Cx. pipiens s.l. with blood-meals from humans, chicken, and sparrow (Passer sp.). We did not detect dengue or chikungunya viruses. However, one Culex poicilipes female was positive for Sindbis virus, 30 pools of Ae. aegypti had cell fusing agent virus (CFAV; infection rate (IR) = 1.27%, 95% CI = 0.87%-1.78%); 11 pools of Ae. aegypti had Aedes flavivirus (AeFV; IR = 0.43%, 95% CI = 0.23%-0.74%); and seven pools of Cx. pipiens s.l. (IR = 0.23%, 95% CI = 0.1%-0.45%) and one pool of Culex annulioris had Culex flavivirus. Sindbis virus, which causes febrile illness in humans, can complicate the diagnosis and prognosis of patients with fever. The presence of Sindbis virus in a single mosquito from a population of mosquitoes with ISFs calls for further investigation into the role ISFs may play in blocking transmission of other arboviruses in this region.en_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.librarianpm2022en_ZA
dc.description.urihttp://www.plosone.orgen_ZA
dc.identifier.citationChiuya, T., Masiga, D.K., Falzon, L.C., Bastos, A.D.S., Fèvre, E.M. & Villinger, J.(2021) A survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenya. PLoS ONE 16(5): e0252369. https://doi.org/10.1371/journal.pone.0252369.en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.other10.1371/journal.pone.0252369
dc.identifier.urihttp://hdl.handle.net/2263/84546
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights© 2021 Chiuya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectAedes aegyptien_ZA
dc.subjectCulex pipiensen_ZA
dc.subjectTick-borne virusen_ZA
dc.subjectLivestock marketsen_ZA
dc.subjectHospitalsen_ZA
dc.subjectInsect-specific flaviviruses (ISFs)en_ZA
dc.subjectMosquitoesen_ZA
dc.subjectKenyaen_ZA
dc.titleA survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenyaen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Chiuya_Survey_2021.pdf
Size:
3.02 MB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: