Quantitative UPLC-MS/MS analysis of obliquumol from Ptaeroxylon obliquum (Thunb.) Radlk. extracts and biological activities of its semi-synthesised derivative ptaeroxylinol

dc.contributor.authorRamadwa, Thanyani Emelton
dc.contributor.authorSelepe, Mamoalosi A.
dc.contributor.authorSonopo, M.S.
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorEloff, Jacobus Nicolaas
dc.date.accessioned2023-06-22T07:55:40Z
dc.date.issued2023-05
dc.description.abstractQuantification of compounds in plant extracts is rarely conducted to determine variation in concentrations of bioactive constituents. The aim of the study was to develop a method using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) to identify and quantify obliquumol (12-O-acetylptaeroxylinol) in Ptaeroxylon obliquum leaves collected from different localities in South Africa. Additionally, biological activity of a semi-synthesized derivative, ptaeroxylinol was investigated. Column chromatography was used to isolate obliquumol from P. obliquum leaves, and thereafter it was saponified to ptaeroxylinol. Ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-qTof-MS) was carried out on the different P. obliquum extracts to quantify obliquumol. A serial microdilution method was used to determine the minimum inhibitory concentration (MIC) against non-pathogenic mycobacteria and fungi. The cytotoxicity was determined using Vero monkey kidney and human liver (C3A) cells. A method was developed to isolate large quantities of obliquumol (0.14%) from dried P. obliquum leaves. The different P. obliquum acetone extracts had variable obliquumol concentrations between 0.1–38.5 µg/mg. Ptaeroxylinol had an MIC as low as 8 µg/mL and 16 µg/mL against Candida albicans ATCC 10,231 and Cryptococcus neoformans, respectively. With an IC50 of 85.7 μg/mL for Vero cells and 126.51 μg/mL for C3A cells, respectively, ptaeroxylinol had low cytotoxicity to the cells tested. A UPLC-MS/MS method was developed to quantify obliquumol content in the P. obliquum acetone extracts. Ptaeroxylinol had good activity against C. albicans (MIC = 8 µg/mL) and it appears that the cleavage of the acetoxy to alcohol group played a role in the antimicrobial activity.en_US
dc.description.departmentChemistryen_US
dc.description.departmentParaclinical Sciencesen_US
dc.description.embargo2024-03-09
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe National Research Foundation (NRF), South Africa.en_US
dc.description.urihttp://www.elsevier.com/locate/sajben_US
dc.identifier.citationRamadwa, T.E., Selepe, M.A., Sonopo, M.S. et al. 2023, 'Quantitative UPLC-MS/MS analysis of obliquumol from Ptaeroxylon obliquum (Thunb.) Radlk. extracts and biological activities of its semi-synthesised derivative ptaeroxylinol', South African Journal of Botany, vol. 155, pp. 35-42, doi : 10.1016/j.sajb.2023.03.006.en_US
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2023.03.006
dc.identifier.urihttp://hdl.handle.net/2263/91180
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2023 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 155, pp. 35-42, 2023, doi : 10.1016/j.sajb.2023.03.00.en_US
dc.subjectUltra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS)en_US
dc.subjectAntifungal activityen_US
dc.subjectAntimycobacterialen_US
dc.subjectCytotoxicityen_US
dc.subjectCryptococcus neoformansen_US
dc.subjectCandida albicansen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleQuantitative UPLC-MS/MS analysis of obliquumol from Ptaeroxylon obliquum (Thunb.) Radlk. extracts and biological activities of its semi-synthesised derivative ptaeroxylinolen_US
dc.typePostprint Articleen_US

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Ramadwa_Quantitative_2023.pdf
Size:
352.23 KB
Format:
Adobe Portable Document Format
Description:
Postprint Article
Loading...
Thumbnail Image
Name:
Ramadwa_QuantitativeSuppl_2023.pdf
Size:
202.12 KB
Format:
Adobe Portable Document Format
Description:
Supplementary Material

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: