Cryopreservation of sheathed third-stage larvae of Nematodirus spathiger
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Date
Authors
Titoy, G.A.P.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
A technique for the cryopreservation of sheathed third-stage larvae of Nematodirus spathiger is described.
It consists of incubating larvae in 5% (v/v) ethylene glycol for 20 min at room temperature,
followed by 1 min of incubation in 40% (v/v) ethylene glycol at 0°C. The average recovery of adult worms
was 28,5% for two adult sheep infected with these larvae.
Description
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Keywords
Veterinary medicine, Cryopreservation, Sheathed third-stage larvae, Nematodirus spathiger
Sustainable Development Goals
Citation
Titoy, GAP 1996, 'Cryopreservation of sheathed third-stage larvae of Nematodirus spathiger’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 1, pp. 63.