Genotoxicity and antigenotoxicity of selected South African indigenous plants
| dc.contributor.author | Makhuvele, Rhulani | |
| dc.contributor.author | Matshoga, R.G. | |
| dc.contributor.author | Antonissen, R. | |
| dc.contributor.author | Pieters, Luc | |
| dc.contributor.author | Verschaeve, Luc | |
| dc.contributor.author | Elgorashi, Esam E. | |
| dc.date.accessioned | 2019-02-21T07:56:48Z | |
| dc.date.available | 2019-02-21T07:56:48Z | |
| dc.date.issued | 2018-01 | |
| dc.description.abstract | Twenty-two plant species extracted with dichloromethane and 90% methanol were investigated for their genotoxicity as well as antigenotoxicity against aflatoxin B1 induced-mutagenicity using the Ames (Salmonella typhimurium strains TA98 and TA100) and Vitotox assays in the presence of S9 rat liver fraction. The results obtained from Ames assay for some plant extracts correlated well with the results obtained from the Vitotox assay. Dichloromethane and methanolic extracts of Helichrysum petiolare, Protea hybrid, Protea roupelliae, Artabotrys brachypetalus (leaves), Friesodielsia obovata, Hexalobus monopetalus, Monanthotaxis caffra, Monodora junodis, Uvaria caffra, Xylopia parviflora, Podocarpus henkellii, Rhoicissus sekhukhuniensis, Podocarpus elongatus and Agapanthus praecox had moderate to strong antimutagenic activities in both Ames and Vitotox assays. The methanolic extract of Annona senegalensis and dichloromethane extract of Podocarpus falcutus also showed antigenotoxic potentials against aflatoxin B1 induced mutagenicity. Methanolic extracts of Xylopia sp., showed a co-mutagenic effect with aflatoxin B1 in the Ames assay (strain TA100). All extracts were not genotoxic in the Vitotox assay in the absence of S9. Plant extracts with promising antimutagenic effects could be used in the form of feed and food supplements as a preventative strategy against aflatoxin B1 induced mutagenicity and carcinogenicity. | en_ZA |
| dc.description.department | Paraclinical Sciences | en_ZA |
| dc.description.librarian | hj2019 | en_ZA |
| dc.description.sponsorship | The National Research Foundation of South Africa (CPRR 87746 and NRF/FWO 87964), the Fund for Scientific Research (FWO – Flanders-Belgium), the Flemish Interuniversity Council (VLIR) and Agricultural Research Council (ARC-OVI). | en_ZA |
| dc.description.uri | http://www.elsevier.com/locate/sajb | en_ZA |
| dc.identifier.citation | Makhuvele, R., Matshoga, R.G., Antonissen, R. et al. 2018, 'Genotoxicity and antigenotoxicity of selected South African indigenous plants', South African Journal of Botany, vol. 114, pp. 89-99. | en_ZA |
| dc.identifier.issn | 0254-6299 (print) | |
| dc.identifier.issn | 1727-9321 (online) | |
| dc.identifier.other | 10.1016/j.sajb.2017.10.016 | |
| dc.identifier.uri | http://hdl.handle.net/2263/68496 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.rights | © 2017 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 114, pp. 89-99, 2018. doi : 10.1016/j.sajb.2017.10.016. | en_ZA |
| dc.subject | Genotoxicity | en_ZA |
| dc.subject | Antigenotoxicity | en_ZA |
| dc.subject | Aflatoxin B-1 | en_ZA |
| dc.subject | Vitotox assay | en_ZA |
| dc.subject | Ames assay | en_ZA |
| dc.subject | Plant extracts | en_ZA |
| dc.subject | Hepatocellular carcinoma | en_ZA |
| dc.subject | Antimutagenicity | en_ZA |
| dc.subject | Mutagenicity | en_ZA |
| dc.subject | Mycotoxins | en_ZA |
| dc.title | Genotoxicity and antigenotoxicity of selected South African indigenous plants | en_ZA |
| dc.type | Postprint Article | en_ZA |