Production and properties of monoclonal antibodies against African horsesickness virus serotype 3
| dc.contributor.author | Van Wyngaardt, Wouter | |
| dc.contributor.author | Du Plessis, D.H. | |
| dc.contributor.author | Van Wyngaardt, Sandra | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.contributor.upauthor | Verschoor, Jan Adrianus | |
| dc.date.accessioned | 2013-11-19T10:59:05Z | |
| dc.date.available | 2013-11-19T10:59:05Z | |
| dc.date.created | 2013 | |
| dc.date.issued | 1992 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | Four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (McAbs) specific for African horsesickness virus (AHSV). Two recognised the major core structural polypeptide, VP7, while one each was specific for the outer capsid proteins, VP2 and VP5. The remainder co-precipitated both VP2 and VP7. An inhibition ELISA and radio-immunoprecipitation revealed two types of co-precipitating McAbs, distinguishable from each other by the different relative amounts of the two proteins they precipitated. Only co-precipitating McAbs reduced the size and number of plaques formed by AHSV on VERO cell monolayers, but even at low dilution did not completely abolish virus infectivity. A McAb specific for VP7 showed potential as a group-reactive diagnostic reagent since guinea pig antisera to all nine serotypes of AHSV, as well as an anti-serotype 4 horse serum and an anti-serotype 3 rabbit serum, inhibited its binding in ELISA to AHSV serotype 3. | en |
| dc.description.librarian | mn2013 | |
| dc.identifier.citation | Van Wyngaardt, W, Du Plessis, DH, Van Wyngaardt, S & Verschoor, JA 1992, 'Production and properties of monoclonal antibodies against African horsesickness virus serotype 3', Onderstepoort Journal of Veterinary Research, vol. 59, no. 2, pp. 129-133. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/32469 | |
| dc.language.iso | en | en |
| dc.publisher | Published by the Agricultural Research Council, Onderstepoort Veterinary Institute. | en |
| dc.rights | © South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | AHSV | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.subject.lcsh | African horse sickness -- South Africa | en |
| dc.title | Production and properties of monoclonal antibodies against African horsesickness virus serotype 3 | en |
| dc.type | Article | en |