Bacterial community in apparently healthy and asymptomatic Eucalyptus trees and those with symptoms of bacterial wilt

Abstract

Ralstonia solanacearum and R. pseudosolanacearum are well-known bacterial plant pathogens that cause significant losses to both ornamental and agricultural plants. It has been suggested that they are not the primary cause of bacterial wilt in Eucalyptus species, but rather are opportunistic, taking advantage of trees predisposed to infection by abiotic and biotic factors. To test this hypothesis, the bacterial community within the vascular tissue of asymptomatic Eucalyptus grandis x E. urophylla trees, and those displaying varying stages of infection in China and Indonesia were compared using 16S rRNA profiling. Asymptomatic trees growing in areas where bacterial infections had never previously been reported to occur were included as controls. Ralstonia species were found within the vascular tissue of both asymptomatic and symptomatic trees, in high abundance. In the control samples, bacterial diversity within the vascular tissue was high with a low abundance of Ralstonia species. The presence of Ralstonia species in asymptomatic and control samples supports the hypothesis that these species are latent and/or opportunistic pathogens in E. grandis x E. urophylla trees.