Whole genome sequence analysis of Brucella spp. from human, livestock, and wildlife in South Africa

dc.contributor.authorMazwi, Koketso Desiree
dc.contributor.authorLekota, Kgaugelo Edward
dc.contributor.authorGlover, Barbara Akofo
dc.contributor.authorKolo, Francis Babaman
dc.contributor.authorHassim, Ayesha
dc.contributor.authorRossouw, Jenny
dc.contributor.authorJonker, Annelize
dc.contributor.authorWojno, Justnya Maria
dc.contributor.authorProfiti, Giuseppe
dc.contributor.authorMartelli, Pier Luigi
dc.contributor.authorCasadio, Rita
dc.contributor.authorZilli, Katiuscia
dc.contributor.authorJanowicz, Anna
dc.contributor.authorMarotta, Francesca
dc.contributor.authorGarofolo, Giuliano
dc.contributor.authorVan Heerden, Henriette
dc.date.accessioned2024-10-30T12:20:54Z
dc.date.available2024-10-30T12:20:54Z
dc.date.issued2024-09
dc.descriptionDATA AVAILABILITY : The genomes data is available on NCBI under project PRJNA-1097985.en_US
dc.description.abstractBrucellosis is an economically important zoonotic disease affecting humans, livestock, and wildlife health globally and especially in Africa. Brucella abortus and B. melitensis have been isolated from human, livestock (cattle and goat), and wildlife (sable) in South Africa (SA) but with little knowledge of the population genomic structure of this pathogen in SA. As whole genome sequencing can assist to differentiate and trace the origin of outbreaks of Brucella spp. strains, the whole genomes of retrospective isolates (n = 19) from previous studies were sequenced. Sequences were analysed using average nucleotide identity (ANI), pangenomics, and whole genome single nucleotide polymorphism (wgSNP) to trace the geographical origin of cases of brucellosis circulating in human, cattle, goats, and sable from different provinces in SA. Pangenomics analysis of B. melitensis (n = 69) and B. abortus (n = 56) was conducted with 19 strains that included B. abortus from cattle (n = 3) and B. melitensis from a human (n = 1), cattle (n = 1), goat (n = 1), Rev1 vaccine strain (n = 1), and sable (n = 12). Pangenomics analysis of B. melitensis genomes, highlighted shared genes, that include 10 hypothetical proteins and genes that encodes for acetyl-coenzyme A synthetase (acs), and acylamidase (aam) amongst the sable genomes. The wgSNP analysis confirmed the B. melitensis isolated from human was more closely related to the goat from the Western Cape Province from the same outbreak than the B. melitensis cattle sample from different cases in the Gauteng Province. The B. melitensis sable strains could be distinguished from the African lineage, constituting their own African sub-clade. The sequenced B. abortus strains clustered in the C2 lineage that is closely related to the isolates from Mozambique and Zimbabwe. This study identified genetically diverse Brucella spp. among various hosts in SA. This study expands the limited known knowledge regarding the presence of B. melitensis in livestock and humans in SA, further building a foundation for future research on the distribution of the Brucella spp. worldwide and its evolutionary background.en_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipSupported by the Belgian Directorate-General for Development Cooperation (DGD) within the DGD-ITM Framework Agreement 4, Gauteng Department of Agriculture and Rural Development GDARD 2016-2017, National Research Foundation, NRF grant 98651 and Erasmus plus. Open access funding provided by University of Pretoria.en_US
dc.description.urihttps://www.springer.com/journal/12275en_US
dc.identifier.citationMazwi, K.D., Lekota, K.E., Glover, B.A. et al. Whole Genome Sequence Analysis of Brucella spp. from Human, Livestock, and Wildlife in South Africa. Journal of Microbiology 2024 Sep; 62(9): 759-773. doi: 10.1007/s12275-024-00155-8.en_US
dc.identifier.issn1225-8873 (print)
dc.identifier.issn1976-3794 (online)
dc.identifier.other10.1007/s12275-024-00155-8
dc.identifier.urihttp://hdl.handle.net/2263/98849
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© 2024. The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectBrucellosisen_US
dc.subjectHumanen_US
dc.subjectLivestocken_US
dc.subjectWildlifeen_US
dc.subjectBrucella abortusen_US
dc.subjectBrucella melitensisen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.subjectWhole genome sequencing (WGS)en_US
dc.titleWhole genome sequence analysis of Brucella spp. from human, livestock, and wildlife in South Africaen_US
dc.typeArticleen_US

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