Fungal diversity and community composition of wheat rhizosphere and non-rhizosphere soils from three different agricultural production regions of South Africa
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Date
Authors
Gqozo, Magalane Pheladi
Bill, Malick
Siyoum, Nazareth Afewerki
Labuschagne, Nico
Korsten, Lise
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Understanding complex interactions among plant genotypes, environmental conditions and microbiome structure provides crucial information for sustainable farming practices towards disease control in agriculture. In this study, fungal diversity and composition in wheat rhizosphere and non-rhizosphere soils were investigated. Special emphasis was placed on pathogenic and beneficial genera. Wheat rhizosphere and non-rhizosphere soil from three different wheat growing regions were analyzed using Illumina high-throughput sequencing. The analysis showed a significant decline in the fungal diversity and richness from non-rhizosphere to rhizosphere soils. Ascomycota and Basidiomycota were the dominant fungal phyla detected in both rhizosphere and non-rhizosphere soils across the three test sites. Genera known to include wheat pathogens detected included Fusarium, Phoma and Colletotrichum genera while, beneficial groups included Trichoderma, Aureobasidium and Acaulospora. The presence of Fusarium was observed to be inversely proportional to that of Aureobasidium, a well-known antagonist of the Fusarium spp. This information could provide new opportunities to explore the potential of manipulating natural fungal antagonistic microorganisms for use in controlling soil-borne pathogenic fungi in wheat.
Description
Supplementary Table A1. Permutational multivariate analysis of variance (PERMANOVA) of main factors tested and their interactions for wheat rhizosphere and non-rhizosphere soil from the same field sites.
Supplementary Table A2. Descriptive statistics on OUT richness (Chao 1) and species diversity (Shannon-Weaver).
Supplementary Fig. A1. Overall relative abundance of fungal class on SST88 and Kariega rhizosphere (R), and non-rhizosphere (NR) soil at Site A, Kariega rhizosphere (R) and non-rhizosphere soil at site B and Eland rhizosphere and non-rhizosphere soil at Site C wheat growing area. SST88_A, SST88 rhizosphere soil at Site A; Kariega_A, Kariega rhizosphere soil at Site A; NR_A, Non- rhizosphere soil at Site A; Kariega_B, Kariega rhizosphere soil at Site B; NR_B, Non- rhizosphere soil at Site B; Eland_C, Eland rhizosphere soil at Site C; NR_C, Non- rhizosphere soil at Site C.
Supplementary Fig. A2. Overall relative abundance of fungal order on cv. SST88 wheat rhizosphere (R) at site A.
Supplementary Fig. A3. Overall relative abundance of fungal order on cv. Kariega wheat rhizosphere (R) at site A.
Supplementary Table A2. Descriptive statistics on OUT richness (Chao 1) and species diversity (Shannon-Weaver).
Supplementary Fig. A1. Overall relative abundance of fungal class on SST88 and Kariega rhizosphere (R), and non-rhizosphere (NR) soil at Site A, Kariega rhizosphere (R) and non-rhizosphere soil at site B and Eland rhizosphere and non-rhizosphere soil at Site C wheat growing area. SST88_A, SST88 rhizosphere soil at Site A; Kariega_A, Kariega rhizosphere soil at Site A; NR_A, Non- rhizosphere soil at Site A; Kariega_B, Kariega rhizosphere soil at Site B; NR_B, Non- rhizosphere soil at Site B; Eland_C, Eland rhizosphere soil at Site C; NR_C, Non- rhizosphere soil at Site C.
Supplementary Fig. A2. Overall relative abundance of fungal order on cv. SST88 wheat rhizosphere (R) at site A.
Supplementary Fig. A3. Overall relative abundance of fungal order on cv. Kariega wheat rhizosphere (R) at site A.
Keywords
Fungal communities, Triticum sativum L., High-throughput ITS sequencing, Microbiome, Soil microbiology
Sustainable Development Goals
Citation
Gqozo, M.P., Bill, M., Siyoum, N. et al. 2020, 'Fungal diversity and community composition of wheat rhizosphere and non-rhizosphere soils from three different agricultural production regions of South Africa', Applied Soil Ecology, vol. 151, art. 103543, pp. 1-9.