In vitro cytotoxicity, antioxidant and anti-inflammatory activities of Pittosporum viridiflorum Sims and Hypoxis colchicifolia Baker used traditionally against cancer in Eastern Cape, South Africa

dc.contributor.authorMadikizela, Balungile
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.emaillyndy.mcgaw@up.ac.zaen_ZA
dc.date.accessioned2020-02-26T13:08:50Z
dc.date.issued2019-11
dc.description.abstractCancer is one of the leading causes of death both in developed and developing countries and was responsible for the loss of 9.6 million lives in 2018 alone. Development of drug resistant strains of cancer has hampered cancer therapy. Worldwide, plants have been reported to play a major role in the traditional treatment of cancer. Irinotecan, paclitaxel, and vincristine are some of the examples of plant derived compounds that are used as anticancer agents. This study evaluated the cytotoxicity, anti-inflammatory, and antioxidant activities of Pittosporum viridiflorum Sims and Hypoxis colchicifolia Baker, two plants used in South African traditional medicine for treating cancer. Acetone, ethanol, and water (hot and cold) extracts were screened against four human cancer lines (breast MCF-7, colorectal Caco-2, lung A549 and cervical Hela) for anticancer potential. Two normal cells; Vero monkey kidney and bovine dermis were used to test for cytotoxicity, and Salmonella typhimurium TA98 and TA100 were used to determine genotoxicity of the samples. The acetone extract of P. viridiflorum had significant cytotoxicity against the cancer cells (MCF7, Caco-2, A549 and Hela) with LC50 values ranging from 3.16–26.87 μg/ml. The same extract had noteworthy anti-inflammatory activity against 15-lipoxygenase with IC50 value = 5.01 μg/ml and was not toxic against Vero and bovine dermis cells nor was it genotoxic. This warrants further extensive study to determine the mechanism of action of anticancer and anti-inflammatory activities as well as to identify specific compounds responsible for the observed activities.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.embargo2020-11-01
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipthe National Research Foundation (NRF), Pretoria, South Africa (NRF-Scarce Skills Postdoctoral Fellowship UID 96848).en_ZA
dc.description.urihttp://www.elsevier.com/locate/sajben_ZA
dc.identifier.citationMadikizela, B. & McGaw, L.J. 2019, 'In vitro cytotoxicity, antioxidant and anti-inflammatory activities of Pittosporum viridiflorum Sims and Hypoxis colchicifolia Baker used traditionally against cancer in Eastern Cape, South Africa', South African Journal of Botany, vol. 126, pp. 250-255.en_ZA
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2019.06.009
dc.identifier.urihttp://hdl.handle.net/2263/73569
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2019 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 126, pp. 250-255, 2019. doi : 10.1016/j.sajb.2019.06.009.en_ZA
dc.subjectAnticanceren_ZA
dc.subjectAnti-inflammatoryen_ZA
dc.subjectAntioxidanten_ZA
dc.subjectCytotoxicity assaysen_ZA
dc.subjectMedicinal plantsen_ZA
dc.subjectMTT assayen_ZA
dc.subjectPittosporum viridiflorumen_ZA
dc.subjectHypoxis colchicifoliaen_ZA
dc.titleIn vitro cytotoxicity, antioxidant and anti-inflammatory activities of Pittosporum viridiflorum Sims and Hypoxis colchicifolia Baker used traditionally against cancer in Eastern Cape, South Africaen_ZA
dc.typePostprint Articleen_ZA

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