Immune responses in a horse inoculated with the VP2 gene of African horsesickness virus

dc.contributor.authorRomito, M.
dc.contributor.authorDu Plessis, D.H.
dc.contributor.authorViljoen, G.J.
dc.contributor.editorVerwoerd, Daniel Wynand
dc.date.accessioned2012-10-08T07:55:06Z
dc.date.available2012-10-08T07:55:06Z
dc.date.created2012
dc.date.issued1999
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en
dc.description.abstractThe ability of a DNA vaccine to elicit an immune response in a horse was evaluated. The outer capsid protein VP2 of African horsesickness virus is known to elicit protective immunity in horses. Reverse transcribed DNA of the gene encoding VP2 was placed under the transcriptional control of the cytomegalovirus immediate-early enhancer/promoter and was injected on several occasions intramuscularly into a horse. Low antibody levels could be detected by ELISA. Antibodies directed against VP2 alone were shown by Western blot while low levels of neutralizing antibodies were detected by a 50% plaque reduction assay. In contrast to a relatively poor humoral response, a significant lymphoproliferative response in the presence of whole virus proteins, as well as a cytotoxic cellular reaction against virus-infected syngeneic target cells was shown.en
dc.description.librarianmn2012en
dc.identifier.citationRomito, M, Du Plessis, DH & Viljoen, GJ 1999, 'Immune responses in a horse inoculated with the VP2 gene of African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 66, no. 2, pp. 139-144.en
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/20083
dc.language.isoenen
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en
dc.subjectVeterinary medicineen
dc.subjectAfrican horsesickness virus (AHSV)en
dc.subjectDNA vaccinesen
dc.subjectVP2 geneen
dc.subject.lcshVeterinary medicine -- South Africa
dc.subject.lcshAfrican horse sickness -- South Africaen
dc.subject.lcshHorses -- Virus diseasesen
dc.titleImmune responses in a horse inoculated with the VP2 gene of African horsesickness virusen
dc.typeArticleen

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