Development and characterization of 13 new, and cross amplification of 3, polymophic nuclear microsattellite loci in the common myna (Acridotheres tristis)

dc.contributor.authorBerthouly-Salazar, Cecile
dc.contributor.authorCassey, Phillip
dc.contributor.authorJansen van Vuuren, Bettine J.
dc.contributor.authorJanse Van Rensburg, Berndt
dc.contributor.authorHui, Cang
dc.contributor.authorGardner, Michael G.
dc.contributor.authorLe Roux, J.J. (Johannes Jacobus)
dc.date.accessioned2014-05-08T07:09:18Z
dc.date.available2014-05-08T07:09:18Z
dc.date.issued2012
dc.description.abstractWe used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.en
dc.description.librarianhb2014en
dc.description.librarianab2014
dc.description.sponsorshipCBS acknowledges support and funding from Stellenbosch University’s DST-NRF-Centre for Invasion Biology. PC is an ARC Future Fellow. CH receives support from the NRF Incentive Programme and the Subcommittee B at Stellenbosch University.en
dc.description.urihttp://link.springer.com/journal/12686en
dc.identifier.citationBerthouly-Salazar, C, Cassey, P, Van Vuuren, BJ, Van Rensburg, BJ, Hui, C, Gardner, MG & Le Roux, JJ 2012, 'Development and characterization of 13 new, and cross amplification of 3, polymophic nuclear microsattellite loci in the common myna (Acridotheres tristis)', Conservation Genetics Resources, vol. 4, no. 3, pp. 621-624.en
dc.identifier.issn1877-7252 (print)
dc.identifier.issn1877-7260 (online)
dc.identifier.other10.1007/s12686-012-9607-8
dc.identifier.urihttp://hdl.handle.net/2263/39726
dc.language.isoenen
dc.publisherSpringeren
dc.rights© Springer Science+Business Media B.V. 2012. The original publication is available at : http://link.springer.com/journal/12686en
dc.subjectAcridotheres tristisen
dc.subjectInvasive speciesen
dc.subjectMicrosatellite locien
dc.subject.lcshMynahsen
dc.subject.lcshSturnidaeen
dc.titleDevelopment and characterization of 13 new, and cross amplification of 3, polymophic nuclear microsattellite loci in the common myna (Acridotheres tristis)en
dc.typePostprint Articleen

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