Improving the specificity of indirect immunofluorescence for the serological diagnosis of bovine anaplasmosis

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Date

Authors

Lawrence, J.A.
Whiteland, A.P.
Kafuwa, P.T.
Njuguna, L.M.

Journal Title

Journal ISSN

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Published by the Agricultural Research Council, Onderstepoort Veterinary Institute

Abstract

Fluorescing inclusion appendages were detected consistently in preparations of Anaplasma centrale and Anaplasma marginale when they were used as antigen in indirect immunofluorescence serological tests for the diagnosis of anaplasmosis in cattle. The presence of the inclusion appendages made it possible to confirm the specificity of the immunofluorescent reaction and to determine end-points with accuracy.

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The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.

Keywords

Veterinary medicine, Bovine anaplasmosis, Diagnosis, Indirect immunofluorescence, Serological, Specificity

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Citation

Lawrence, JA, Whiteland, AP, Kafuwa, PT & Njuguna, LM 1996, 'Improving the specificity of indirect immunofluorescence for the serological diagnosis of bovine anaplasmosis’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 1, pp. 53-55.