Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont

dc.contributor.authorVan Elst, Daan
dc.contributor.authorVan Wyk, Braam
dc.contributor.authorSchultz, Anitra
dc.contributor.authorPrinsen, Els
dc.contributor.emailbraam.vanwyk@up.ac.zaen_US
dc.date.accessioned2013-05-06T07:00:02Z
dc.date.available2013-05-06T07:00:02Z
dc.date.issued2013-01
dc.description.abstractPlant poisoning of livestock is responsible for considerable economic losses in southern Africa. Six plant species of the Rubiaceae family are known to cause gousiekte, a cardiac syndrome of ruminants induced by ingestion of the toxic compound pavettamine. Progress in understanding the aetiology of this disease is largely hampered by the variable toxicity of the plants and the absence of a quantification method for pavettamine. The pavettamine concentration in leaf samples of Fadogia homblei, a known gousiekte causing plant, was analyzed by massspectrometry. In the most apical leaf pair, the highest concentration of pavettamine was detected. Distal leaves contained progressively less pavettamine. Besides a significant amount of free pavettamine, most pavettamine was found to occur in a conjugated form. To which molecules the pavettamine is conjugated remains unknown as is the function of conjugated pavettamine in the development of gousiekte. All know gousiekte-causing plants contain symbiotic bacteria in their leaves; it was hypothesized that these bacteria might be involved in the production of pavettamine. However, analysis of in vitro cultures of the Fadogia homblei endosymbiont revealed no production of pavettamine. Pavettamine is therefore not produced by the bacteria alone. It is either the product of the interaction with the plant or solely produced by the host.en_US
dc.description.librarianhb2013en_US
dc.description.sponsorshipThis research was supported financially by the Fund for Scientific Research, Flanders (F.W.O., G.0343.09N). D.V.E. holds a Ph.D. research grant from the Fund for Scientific Research, Flanders (F.W.O., no. 1.1.722.10.N.00).en_US
dc.description.urihttp://www.journals.elsevier.com/phytochemistry/en_US
dc.identifier.citationVan Elst, D, Van Wyk, B, Schultz, A & Prinsen, E 2013, 'Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont', Phytochemistry, vol. 85, no. 1, pp. 92-98.en_US
dc.identifier.issn0031-9422 (print)
dc.identifier.issn1873-3700 (online)
dc.identifier.other10.1016/j.phytochem.2012.08.025
dc.identifier.urihttp://hdl.handle.net/2263/21443
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, vol. 85, no. 1, 2013, DOI : 10.1016/j.phytochem.2012.08.025en_US
dc.subjectFadogia hombleien_US
dc.subjectRubiaceaeen_US
dc.subjectBurkholderiaen_US
dc.subjectEndosymbionten_US
dc.subjectMass-spectrometryen_US
dc.subjectPavettamineen_US
dc.subjectGousiekteen_US
dc.titleProduction of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbionten_US
dc.typePostprint Articleen_US

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